Additive effect of mPer1 and mPer2 antisense oligonucleotides on light-induced phase shift

Hisanori Wakamatsu, Satomi Takahashi, Takahiro Moriya, Shin Ichi T. Inouye, Hitoshi Okamura, Masashi Akiyama, Shigenobu Shibata

Research output: Contribution to journalArticle

85 Citations (Scopus)

Abstract

It is well known that light induces both mPer1 and mPer2 mRNA in the suprachiasmatic nucleus. We have reported that mPer1 antisense oligonucleotides (ODNs) inhibited the light-induced phase delays of mouse locomotor rhythm. In this study, we asked whether both or either mPer1 or mPer2 expression is necessary to induce the phase shift. We examined the effects of inhibition of mRNA expression on light-induced phase delays of mouse circadian behavior rhythm. Light-induced phase delays were moderately attenuated by microinjection of mPer1 or mPer2 antisense ODN, but not by mPer3 antisense or mPer1, mPer2 scrambled ODNs, whereas following simultaneous injection of both mPer1 and mPer2 antisense ODNs they disappeared. The present results suggest that acute induction of mPer1 and mPer2 gene play an additive effect on photic entrainment.

Original languageEnglish
Pages (from-to)127-131
Number of pages5
JournalNeuroReport
Volume12
Issue number1
DOIs
Publication statusPublished - 2001 Jan 22

Keywords

  • Antisense oligonucleotides
  • Circadian clock
  • MPer
  • Suprachiasmatic nucleus

ASJC Scopus subject areas

  • Neuroscience(all)

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