TY - JOUR
T1 - Adequate taylor couette flow-mediated shear stress is useful for dissociating human iPS cell-derived cell aggregates
AU - Matsuura, Katsuhisa
AU - Wada, Masanori
AU - Sakaguchi, Katsuhisa
AU - Matsuhashi, Yuki
AU - Shimizu, Tatsuya
N1 - Funding Information:
We thank S. Sugiyama, and M. Matsuda for their excellent technical assistance. We thank Prof. Y. Sawa in Osaka University, Japan and Prof. Y. Yoshida in Kyoto University, Japan for the fruitful collaboration for the development of cardiac differentiation protocol. We thank S. Bou-Ghannam for English proofreading of the manuscript. This work was funded by a grant from Projects for Technological Development in Research Center Network for Realization of Regenerative Medicine of the Japan Science and Technology Agency, JST and Japan Agency for Medical Research and Development, AMED (Grant Number JP17bm0404015 ).
Publisher Copyright:
© 2019
PY - 2019/12/15
Y1 - 2019/12/15
N2 - Pluripotent stem cell including induced pluripotent stem cells (iPSC) are promising cell sources for regenerative medicine and for three-dimensional suspension culture technologies which may enable the generation of robust numbers of desired cells through cell aggregation. Although manual procedure is widely used for dissociating cell aggregates, the development of non-manual procedures using devices will contribute to efficient cell manufacturing. In the present study, we developed novel cell aggregate dissociation devices with a rotating cylinder inside based on taylor couette flow-mediated shear stress. The shear stress can be increased according to an increase in the size of the rotating cylinder inside the devices and the rotation rate. Adequate device size and suitable rotation rate efficiently dissociated cell aggregates after the undifferentiated expansion and the cardiac differentiation of human iPSC. These finding suggest that non-manual device procedure might be useful for harvesting single cells from human iPSC-derived cell aggregates.
AB - Pluripotent stem cell including induced pluripotent stem cells (iPSC) are promising cell sources for regenerative medicine and for three-dimensional suspension culture technologies which may enable the generation of robust numbers of desired cells through cell aggregation. Although manual procedure is widely used for dissociating cell aggregates, the development of non-manual procedures using devices will contribute to efficient cell manufacturing. In the present study, we developed novel cell aggregate dissociation devices with a rotating cylinder inside based on taylor couette flow-mediated shear stress. The shear stress can be increased according to an increase in the size of the rotating cylinder inside the devices and the rotation rate. Adequate device size and suitable rotation rate efficiently dissociated cell aggregates after the undifferentiated expansion and the cardiac differentiation of human iPSC. These finding suggest that non-manual device procedure might be useful for harvesting single cells from human iPSC-derived cell aggregates.
KW - 3D suspension culture
KW - Cell aggregate dissociation device
KW - Taylor couette flow
KW - iPS cell
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U2 - 10.1016/j.reth.2019.04.006
DO - 10.1016/j.reth.2019.04.006
M3 - Article
AN - SCOPUS:85064647183
SN - 2352-3204
VL - 12
SP - 6
EP - 13
JO - Regenerative Therapy
JF - Regenerative Therapy
ER -
