Adrenergic regulation of clock gene expression in mouse liver

Hideyuki Terazono, Tatsushi Mutoh, Shun Yamaguchi, Masaki Kobayashi, Masashi Akiyama, Rhyuta Udo, Shigehiro Ohdo, Hitoshi Okamura, Shigenobu Shibata

    Research output: Contribution to journalArticle

    201 Citations (Scopus)

    Abstract

    A main oscillator in the suprachiasmatic nucleus (SCN) conveys circadian information to the peripheral clock systems for the regulation of fundamental physiological functions. Although polysynaptic autonomic neural pathways between the SCN and the liver were observed in rats, whether activation of the sympathetic nervous system entrains clock gene expression in the liver has yet to be understood. To assess sympathetic innervation from the SCN to liver tissue, we investigated whether injection of adrenaline/ noradrenaline (epinephrine/norepinephrine) or sympathetic nerve stimulation could induce mPer gene expression in mouse liver. Acute administration of adrenaline or noradrenaline increased mPer1 but not mPer2 expression in the liver of mice in vivo and in hepatic slices in vitro. Electrical stimulation of the sympathetic nerves or adrenaline injection caused an elevation of bioluminescence in the liver area of transgenic mice carrying mPer1 promoterluciferase. Under a light-dark cycle, destruction of the SCN flattened the daily rhythms of not only mPer1, mPer2, and mBmal1 genes but also noradrenaline content in the liver. Daily injection of adrenaline, administered at a fixed time for 6 days, recovered oscillations of mPer2 and mBmall gene expression in the liver of mice with SCN lesion on day 7. Sympathetic nerve denervation by 6-hydroxydopamine flattened the daily rhythm of mPer1 and mPer2 gene expression. Thus, on the basis of the present results, activation of the sympathetic nerves through noradrenaline and/or adrenaline release was a factor controlling the peripheral clock.

    Original languageEnglish
    Pages (from-to)6795-6800
    Number of pages6
    JournalProceedings of the National Academy of Sciences of the United States of America
    Volume100
    Issue number11
    DOIs
    Publication statusPublished - 2003 May 27

    Fingerprint

    Gene Expression Regulation
    Adrenergic Agents
    Suprachiasmatic Nucleus
    Epinephrine
    Liver
    Norepinephrine
    Gene Expression
    Injections
    Autonomic Pathways
    Neural Pathways
    Sympathectomy
    Oxidopamine
    Sympathetic Nervous System
    Photoperiod
    Transgenic Mice
    Electric Stimulation

    ASJC Scopus subject areas

    • Genetics
    • General

    Cite this

    Adrenergic regulation of clock gene expression in mouse liver. / Terazono, Hideyuki; Mutoh, Tatsushi; Yamaguchi, Shun; Kobayashi, Masaki; Akiyama, Masashi; Udo, Rhyuta; Ohdo, Shigehiro; Okamura, Hitoshi; Shibata, Shigenobu.

    In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, No. 11, 27.05.2003, p. 6795-6800.

    Research output: Contribution to journalArticle

    Terazono, H, Mutoh, T, Yamaguchi, S, Kobayashi, M, Akiyama, M, Udo, R, Ohdo, S, Okamura, H & Shibata, S 2003, 'Adrenergic regulation of clock gene expression in mouse liver', Proceedings of the National Academy of Sciences of the United States of America, vol. 100, no. 11, pp. 6795-6800. https://doi.org/10.1073/pnas.0936797100
    Terazono, Hideyuki ; Mutoh, Tatsushi ; Yamaguchi, Shun ; Kobayashi, Masaki ; Akiyama, Masashi ; Udo, Rhyuta ; Ohdo, Shigehiro ; Okamura, Hitoshi ; Shibata, Shigenobu. / Adrenergic regulation of clock gene expression in mouse liver. In: Proceedings of the National Academy of Sciences of the United States of America. 2003 ; Vol. 100, No. 11. pp. 6795-6800.
    @article{0131f79f03d249748b6f9d1fbe0e8938,
    title = "Adrenergic regulation of clock gene expression in mouse liver",
    abstract = "A main oscillator in the suprachiasmatic nucleus (SCN) conveys circadian information to the peripheral clock systems for the regulation of fundamental physiological functions. Although polysynaptic autonomic neural pathways between the SCN and the liver were observed in rats, whether activation of the sympathetic nervous system entrains clock gene expression in the liver has yet to be understood. To assess sympathetic innervation from the SCN to liver tissue, we investigated whether injection of adrenaline/ noradrenaline (epinephrine/norepinephrine) or sympathetic nerve stimulation could induce mPer gene expression in mouse liver. Acute administration of adrenaline or noradrenaline increased mPer1 but not mPer2 expression in the liver of mice in vivo and in hepatic slices in vitro. Electrical stimulation of the sympathetic nerves or adrenaline injection caused an elevation of bioluminescence in the liver area of transgenic mice carrying mPer1 promoterluciferase. Under a light-dark cycle, destruction of the SCN flattened the daily rhythms of not only mPer1, mPer2, and mBmal1 genes but also noradrenaline content in the liver. Daily injection of adrenaline, administered at a fixed time for 6 days, recovered oscillations of mPer2 and mBmall gene expression in the liver of mice with SCN lesion on day 7. Sympathetic nerve denervation by 6-hydroxydopamine flattened the daily rhythm of mPer1 and mPer2 gene expression. Thus, on the basis of the present results, activation of the sympathetic nerves through noradrenaline and/or adrenaline release was a factor controlling the peripheral clock.",
    author = "Hideyuki Terazono and Tatsushi Mutoh and Shun Yamaguchi and Masaki Kobayashi and Masashi Akiyama and Rhyuta Udo and Shigehiro Ohdo and Hitoshi Okamura and Shigenobu Shibata",
    year = "2003",
    month = "5",
    day = "27",
    doi = "10.1073/pnas.0936797100",
    language = "English",
    volume = "100",
    pages = "6795--6800",
    journal = "Proceedings of the National Academy of Sciences of the United States of America",
    issn = "0027-8424",
    number = "11",

    }

    TY - JOUR

    T1 - Adrenergic regulation of clock gene expression in mouse liver

    AU - Terazono, Hideyuki

    AU - Mutoh, Tatsushi

    AU - Yamaguchi, Shun

    AU - Kobayashi, Masaki

    AU - Akiyama, Masashi

    AU - Udo, Rhyuta

    AU - Ohdo, Shigehiro

    AU - Okamura, Hitoshi

    AU - Shibata, Shigenobu

    PY - 2003/5/27

    Y1 - 2003/5/27

    N2 - A main oscillator in the suprachiasmatic nucleus (SCN) conveys circadian information to the peripheral clock systems for the regulation of fundamental physiological functions. Although polysynaptic autonomic neural pathways between the SCN and the liver were observed in rats, whether activation of the sympathetic nervous system entrains clock gene expression in the liver has yet to be understood. To assess sympathetic innervation from the SCN to liver tissue, we investigated whether injection of adrenaline/ noradrenaline (epinephrine/norepinephrine) or sympathetic nerve stimulation could induce mPer gene expression in mouse liver. Acute administration of adrenaline or noradrenaline increased mPer1 but not mPer2 expression in the liver of mice in vivo and in hepatic slices in vitro. Electrical stimulation of the sympathetic nerves or adrenaline injection caused an elevation of bioluminescence in the liver area of transgenic mice carrying mPer1 promoterluciferase. Under a light-dark cycle, destruction of the SCN flattened the daily rhythms of not only mPer1, mPer2, and mBmal1 genes but also noradrenaline content in the liver. Daily injection of adrenaline, administered at a fixed time for 6 days, recovered oscillations of mPer2 and mBmall gene expression in the liver of mice with SCN lesion on day 7. Sympathetic nerve denervation by 6-hydroxydopamine flattened the daily rhythm of mPer1 and mPer2 gene expression. Thus, on the basis of the present results, activation of the sympathetic nerves through noradrenaline and/or adrenaline release was a factor controlling the peripheral clock.

    AB - A main oscillator in the suprachiasmatic nucleus (SCN) conveys circadian information to the peripheral clock systems for the regulation of fundamental physiological functions. Although polysynaptic autonomic neural pathways between the SCN and the liver were observed in rats, whether activation of the sympathetic nervous system entrains clock gene expression in the liver has yet to be understood. To assess sympathetic innervation from the SCN to liver tissue, we investigated whether injection of adrenaline/ noradrenaline (epinephrine/norepinephrine) or sympathetic nerve stimulation could induce mPer gene expression in mouse liver. Acute administration of adrenaline or noradrenaline increased mPer1 but not mPer2 expression in the liver of mice in vivo and in hepatic slices in vitro. Electrical stimulation of the sympathetic nerves or adrenaline injection caused an elevation of bioluminescence in the liver area of transgenic mice carrying mPer1 promoterluciferase. Under a light-dark cycle, destruction of the SCN flattened the daily rhythms of not only mPer1, mPer2, and mBmal1 genes but also noradrenaline content in the liver. Daily injection of adrenaline, administered at a fixed time for 6 days, recovered oscillations of mPer2 and mBmall gene expression in the liver of mice with SCN lesion on day 7. Sympathetic nerve denervation by 6-hydroxydopamine flattened the daily rhythm of mPer1 and mPer2 gene expression. Thus, on the basis of the present results, activation of the sympathetic nerves through noradrenaline and/or adrenaline release was a factor controlling the peripheral clock.

    UR - http://www.scopus.com/inward/record.url?scp=0038651005&partnerID=8YFLogxK

    UR - http://www.scopus.com/inward/citedby.url?scp=0038651005&partnerID=8YFLogxK

    U2 - 10.1073/pnas.0936797100

    DO - 10.1073/pnas.0936797100

    M3 - Article

    VL - 100

    SP - 6795

    EP - 6800

    JO - Proceedings of the National Academy of Sciences of the United States of America

    JF - Proceedings of the National Academy of Sciences of the United States of America

    SN - 0027-8424

    IS - 11

    ER -