Adsorption mechanism of ribosomal protein L2 onto a silica surface: A molecular dynamics simulation study

Ryo Tosaka, Hideaki Yamamoto, Iwao Ohdomari, Takanobu Watanabe

    Research output: Contribution to journalArticle

    29 Citations (Scopus)

    Abstract

    A large-scale molecular dynamics simulation was carried out in order to investigate the adsorption mechanism of ribosomal protein L2 (RPL2) onto a silica surface at various pH values. RPL2 is a constituent protein of the 50S large ribosomal subunit, and a recent experimental report showed that it adsorbs strongly to silica surfaces and that it can be used to immobilize proteins on silica surfaces. The simulation results show that RPL2, especially domains 1 (residues 1-60) and 3 (residues 203-273), adsorbed more tightly to the silica surface above pH 7. We found that a major driving force for the adsorption of RPL2 onto the silica surface is the electrostatic interaction and that the structural flexibility of domains 1 and 3 may further contribute to the high affinity.

    Original languageEnglish
    Pages (from-to)9950-9955
    Number of pages6
    JournalLangmuir
    Volume26
    Issue number12
    DOIs
    Publication statusPublished - 2010 Jun 15

    Fingerprint

    Molecular Dynamics Simulation
    Silicon Dioxide
    Adsorption
    Molecular dynamics
    Silica
    molecular dynamics
    silicon dioxide
    proteins
    Proteins
    adsorption
    Computer simulation
    simulation
    Large Ribosome Subunits
    Coulomb interactions
    Static Electricity
    affinity
    ribosomal protein L2
    flexibility
    electrostatics
    interactions

    ASJC Scopus subject areas

    • Electrochemistry
    • Condensed Matter Physics
    • Surfaces and Interfaces
    • Materials Science(all)
    • Spectroscopy
    • Medicine(all)

    Cite this

    Adsorption mechanism of ribosomal protein L2 onto a silica surface : A molecular dynamics simulation study. / Tosaka, Ryo; Yamamoto, Hideaki; Ohdomari, Iwao; Watanabe, Takanobu.

    In: Langmuir, Vol. 26, No. 12, 15.06.2010, p. 9950-9955.

    Research output: Contribution to journalArticle

    Tosaka, Ryo ; Yamamoto, Hideaki ; Ohdomari, Iwao ; Watanabe, Takanobu. / Adsorption mechanism of ribosomal protein L2 onto a silica surface : A molecular dynamics simulation study. In: Langmuir. 2010 ; Vol. 26, No. 12. pp. 9950-9955.
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