Affinity adsorption separation of mutagenic molecules by polyacrylamide hydrogels comprising double-stranded DNA

Daisuke Umeno, Takeshi Kano, Mizuo Maeda

Research output: Contribution to journalArticlepeer-review

30 Citations (Scopus)

Abstract

Polyacrylamide hydrogels comprising double-stranded DNA were synthesized and applied as the absorbent for DNA-binding mutagenic molecules. Vinyl groups were immobilized on salmon sperm DNA via photoreaction between DNA and a vinyl derivative of psoralen. The resulting DNA macromonomer was fed into the polymerization system of acrylamide and N,N'-methylenebisacrylamide to give a hybrid hydrogel between DNA and polyacrylamide. Adsorption functions of the DNA hydrogel were studied for a variety of DNA-binding drugs and dyes. Copyright (C) 1998 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)101-108
Number of pages8
JournalAnalytica Chimica Acta
Volume365
Issue number1-3
DOIs
Publication statusPublished - 1998 Jun 5
Externally publishedYes

Keywords

  • Affinity separation
  • Double-stranded DNA
  • Hybrid material
  • Mutagenic molecules
  • Polyacrylamide gel
  • Psoralen

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Environmental Chemistry
  • Spectroscopy

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