Affinity capillary electrophoresis with a DNA-nanoparticle conjugate as a new tool for genotyping

Ken Adachi, Naohiro Noda, Makoto Nakashige, Satoshi Tsuneda, Takahiro Kanagawa

    Research output: Contribution to journalArticle

    17 Citations (Scopus)

    Abstract

    We have developed a novel method for genotyping based on free solution affinity capillary electrophoresis. We prepared DNA-nanoparticle conjugates by mixing biotin-modified DNA and NeutrAvidin-modified polystyrene nanoparticles; this mixture was then injected into a capillary. Subsequently, we injected the fluorescent-labeled sample DNAs into the capillary, applied the voltage, increased its temperature after 7 min, and detected the fluorescence at its anodic end. This novel method was applied for genotyping human c-K-ras, and the three genotypes were definitely distinguishable with high reproducibility. This method can be easily automated, and it is useful for high-throughput gene mutation analysis.

    Original languageEnglish
    Pages (from-to)127-131
    Number of pages5
    JournalJournal of Chromatography A
    Volume1109
    Issue number2
    DOIs
    Publication statusPublished - 2006 Mar 24

    Fingerprint

    Capillary electrophoresis
    Capillary Electrophoresis
    Nanoparticles
    DNA
    Polystyrenes
    Biotin
    Genes
    Fluorescence
    Genotype
    Throughput
    Mutation
    Temperature
    Electric potential

    Keywords

    • Affinity capillary electrophoresis
    • Free solution affinity capillary electrophoresis
    • Gene point mutation
    • Genotyping
    • Nanoparticle

    ASJC Scopus subject areas

    • Analytical Chemistry

    Cite this

    Affinity capillary electrophoresis with a DNA-nanoparticle conjugate as a new tool for genotyping. / Adachi, Ken; Noda, Naohiro; Nakashige, Makoto; Tsuneda, Satoshi; Kanagawa, Takahiro.

    In: Journal of Chromatography A, Vol. 1109, No. 2, 24.03.2006, p. 127-131.

    Research output: Contribution to journalArticle

    Adachi, Ken ; Noda, Naohiro ; Nakashige, Makoto ; Tsuneda, Satoshi ; Kanagawa, Takahiro. / Affinity capillary electrophoresis with a DNA-nanoparticle conjugate as a new tool for genotyping. In: Journal of Chromatography A. 2006 ; Vol. 1109, No. 2. pp. 127-131.
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