Affinity capillary electrophoresis with a DNA-nanoparticle conjugate as a new tool for genotyping

Ken Adachi, Naohiro Noda, Makoto Nakashige, Satoshi Tsuneda, Takahiro Kanagawa

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

We have developed a novel method for genotyping based on free solution affinity capillary electrophoresis. We prepared DNA-nanoparticle conjugates by mixing biotin-modified DNA and NeutrAvidin-modified polystyrene nanoparticles; this mixture was then injected into a capillary. Subsequently, we injected the fluorescent-labeled sample DNAs into the capillary, applied the voltage, increased its temperature after 7 min, and detected the fluorescence at its anodic end. This novel method was applied for genotyping human c-K-ras, and the three genotypes were definitely distinguishable with high reproducibility. This method can be easily automated, and it is useful for high-throughput gene mutation analysis.

Original languageEnglish
Pages (from-to)127-131
Number of pages5
JournalJournal of Chromatography A
Volume1109
Issue number2
DOIs
Publication statusPublished - 2006 Mar 24

Keywords

  • Affinity capillary electrophoresis
  • Free solution affinity capillary electrophoresis
  • Gene point mutation
  • Genotyping
  • Nanoparticle

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

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