Affinity separation of messenger RNA by thermo-responsive polymer carrying oligo(dT).

T. Mori, F. Oda, D. Umeno, M. Murata, M. Maeda

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

The conjugate between oligo(dT)16 and thermo-responsive polymer, poly(N-isopropylacrylamide), was prepared for isolation of poly(A)+ RNA from total RNA. The hybridization reaction between the conjugate and poly(A) (average length: 320 base) was equilibrated in 10 min, and all the poly(A) (16 nmol base for 24 nmol base of conjugate) was precipitated when raising the solution temperature to 35 degrees C. The precipitate was dissolved in water, and poly(A) was dissociated from the conjugate by heating to 65 degrees C. This separation system was successfully applied to the isolation of poly(A)+ RNA from total RNA.

Original languageEnglish
Pages (from-to)55-56
Number of pages2
JournalNucleic acids symposium series
Issue number42
DOIs
Publication statusPublished - 1999
Externally publishedYes

ASJC Scopus subject areas

  • Medicine(all)

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