Age- and region-specific expressions of the messenger RNAs encoding for steroidogenic enzymes P450scc, P50c17 and 3β-HSD in the postnatal rat brain

Chie Kohchi, Kazuyoshi Ukena, Kazuyoshi Tsutsui

Research output: Contribution to journalArticle

110 Citations (Scopus)

Abstract

Neurosteroids are now known to be synthesized de novo in the nervous system through mechanisms at least partly independent of peripheral steroidogenic glands. In mammals, the presence of the cholesterol side-chain cleavage enzyme (cytochrome P450scc) and the enzyme 3β-hydroxysteroid dehydrogenase/Δ54-isomerase (3β-HSD) has been well established in the brain, whereas limited information has been available on the enzyme 17α- hydroxylase/c17, 20-lyase (cytochrome P450c17), which converts pregnenolone to dehydroepiandrosterone, one of the most abundant neurosteroids. In addition, little is known regarding developmental changes in these steroidogenic enzymes during postnatal life. Thus, the pathway of neurosteroid formation in the brain is still incomplete. Therefore, we examined expressions of the messenger RNAs (mRNAs) encoding for three key enzymes, P450scc, P450c17 and 3β-HSD, in the rat brain at different postnatal ages using RT-PCR analysis. The expression of P450scc mRNA was found throughout the brain at the same level, while the 3β-HSD mRNA expression was higher in the cerebellum and cerebrum than in other brain regions. The P450c17 mRNA was highly expressed in the mesencephalon. On the other hand, higher expressions of the cerebellar and cerebral 3β-HSD mRNAs were observed only in neonatal life. In contrast, the expression of P450scc mRNA was relatively constant during neonatal life and in adulthood. A similar constant expression of the P450c17 mRNA was evident in the mesencephalon. Serial Southern hybridization in this study confirmed the specific mRNA expression corresponding to each enzyme. These results suggest that in the postnatal rat the expression of 3β-HSD or P450c17 mRNA may be age- or region-dependent, unlike the P450scc mRNA expression.

Original languageEnglish
Pages (from-to)233-238
Number of pages6
JournalBrain Research
Volume801
Issue number1-2
DOIs
Publication statusPublished - 1998 Aug 10
Externally publishedYes

Fingerprint

3-Hydroxysteroid Dehydrogenases
Isomerases
Messenger RNA
Brain
Enzymes
Cholesterol Side-Chain Cleavage Enzyme
Neurotransmitter Agents
Mesencephalon
Pregnenolone
Lyases
Dehydroepiandrosterone
Cerebrum
Cytochromes
Mixed Function Oxygenases
Cerebellum
Nervous System
Mammals

Keywords

  • 17α-Hydroxylase/c17, 20- lyase
  • 3β-Hydroxysteroid dehydrogenase/Δ-Δ-isomerase
  • Cholesterol side-chain cleavage enzyme
  • Messenger RNA expression
  • Neurosteroids
  • Postnatal life
  • Rat brain
  • Reverse transcription- polymerase chain reaction

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Age- and region-specific expressions of the messenger RNAs encoding for steroidogenic enzymes P450scc, P50c17 and 3β-HSD in the postnatal rat brain. / Kohchi, Chie; Ukena, Kazuyoshi; Tsutsui, Kazuyoshi.

In: Brain Research, Vol. 801, No. 1-2, 10.08.1998, p. 233-238.

Research output: Contribution to journalArticle

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abstract = "Neurosteroids are now known to be synthesized de novo in the nervous system through mechanisms at least partly independent of peripheral steroidogenic glands. In mammals, the presence of the cholesterol side-chain cleavage enzyme (cytochrome P450scc) and the enzyme 3β-hydroxysteroid dehydrogenase/Δ5-Δ4-isomerase (3β-HSD) has been well established in the brain, whereas limited information has been available on the enzyme 17α- hydroxylase/c17, 20-lyase (cytochrome P450c17), which converts pregnenolone to dehydroepiandrosterone, one of the most abundant neurosteroids. In addition, little is known regarding developmental changes in these steroidogenic enzymes during postnatal life. Thus, the pathway of neurosteroid formation in the brain is still incomplete. Therefore, we examined expressions of the messenger RNAs (mRNAs) encoding for three key enzymes, P450scc, P450c17 and 3β-HSD, in the rat brain at different postnatal ages using RT-PCR analysis. The expression of P450scc mRNA was found throughout the brain at the same level, while the 3β-HSD mRNA expression was higher in the cerebellum and cerebrum than in other brain regions. The P450c17 mRNA was highly expressed in the mesencephalon. On the other hand, higher expressions of the cerebellar and cerebral 3β-HSD mRNAs were observed only in neonatal life. In contrast, the expression of P450scc mRNA was relatively constant during neonatal life and in adulthood. A similar constant expression of the P450c17 mRNA was evident in the mesencephalon. Serial Southern hybridization in this study confirmed the specific mRNA expression corresponding to each enzyme. These results suggest that in the postnatal rat the expression of 3β-HSD or P450c17 mRNA may be age- or region-dependent, unlike the P450scc mRNA expression.",
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