Age-dependent preferential dense-core vesicle exocytosis in neuroendocrine cells revealed by newly developed monomeric fluorescent timer protein

Takashi Tsuboi, Tetsuya Kitaguchi, Satoshi Karasawa, Mitsunori Fukuda, Atsushi Miyawaki

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Although it is evident that only a few secretory vesicles accumulating in neuroendocrine cells are qualified to fuse with the plasma membrane and release their contents to the extracellular space, the molecular mechanisms that regulate their exocytosis are poorly understood. For example, it has been controversial whether secretory vesicles are exocytosed randomly or preferentially according to their age. Using a newly developed protein-based fluorescent timer, monomeric Kusabira Green Orange (mK-GO), which changes color with a predictable time course, here we show that small GTPase Rab27A effectors regulate age-dependent exocytosis of secretory vesicles in PC12 cells. When the vesicles were labeled with mK-GO-tagged neuropeptide Y or tissue-type plasminogen activator, punctate structures with green or red fluorescence were observed. Application of high [K+] stimulation induced exocytosis of new (green) fluorescent secretory vesicles but not of old (red) vesicles. Overexpression or depletion of rabphilin and synaptotagmin-like protein4-a (Slp4-a), which regulate exocytosis positively and negatively, respectively, disturbed the age-dependent exocytosis of the secretory vesicles in different manners. Our results suggest that coordinate functions of the two effectors of Rab27A, rabphilin and Slp4-a, are required for regulated secretory pathway.

Original languageEnglish
Pages (from-to)87-94
Number of pages8
JournalMolecular Biology of the Cell
Volume21
Issue number1
DOIs
Publication statusPublished - 2010 Jan 1
Externally publishedYes

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Neuroendocrine Cells
Exocytosis
Secretory Vesicles
Synaptotagmins
Proteins
Monomeric GTP-Binding Proteins
Secretory Pathway
Neuropeptide Y
PC12 Cells
Extracellular Space
Tissue Plasminogen Activator
Color
Fluorescence
Cell Membrane

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

Age-dependent preferential dense-core vesicle exocytosis in neuroendocrine cells revealed by newly developed monomeric fluorescent timer protein. / Tsuboi, Takashi; Kitaguchi, Tetsuya; Karasawa, Satoshi; Fukuda, Mitsunori; Miyawaki, Atsushi.

In: Molecular Biology of the Cell, Vol. 21, No. 1, 01.01.2010, p. 87-94.

Research output: Contribution to journalArticle

Tsuboi, Takashi ; Kitaguchi, Tetsuya ; Karasawa, Satoshi ; Fukuda, Mitsunori ; Miyawaki, Atsushi. / Age-dependent preferential dense-core vesicle exocytosis in neuroendocrine cells revealed by newly developed monomeric fluorescent timer protein. In: Molecular Biology of the Cell. 2010 ; Vol. 21, No. 1. pp. 87-94.
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