Age-related effects of fasting on ketone body production during lipolysis in rats

Yuriko Higashino-Matsui, Ken Shirato, Yuko Suzuki, Yu Kawashima, Yui Someya, Shogo Sato, Akira Shiraishi, Manabu Jinde, Akiko Matsumoto, Hisashi Ideno, Kaoru Tachiyashiki, Kazuhiko Imaizumi

    Research output: Contribution to journalArticle

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    Abstract

    Objective The age-related effects of fasting on lipolysis, the production of ketone bodies, and plasma insulin levels were studied in male 3-, 8-, and 32-week-old Sprague- Dawley rats. Methods The rats were divided into fasting and control groups. The 3-, 8- and 32-week-old rats tolerated fasting for 2, 5, and 12 days, respectively. Results Fasting markedly reduced the weights of perirenal and periepididymal white adipose tissues in rats in the three age groups. The mean rates of reduction in both these adipose tissue weights during fasting periods were higher in the order of 3>8>32-week-old rats. Fasting transiently increased plasma free fatty acid (FFA), total ketone body, β-hydroxybutyrate, and acetoacetate concentrations in the rats in the three age groups. However, plasma FFA, total ketone body, β-hydroxybutyrate, and acetoacetate concentrations in the 3-week-old rats reached maximal peak within 2 days after the onset of fasting, although these concentrations in the 8- and 32-week-old rats took more than 2 days to reach the maximal peak. By contrast, the augmentation of plasma FFA, total ketone body, β-hydroxybutyrate, and acetoacetate concentrations in the rats in the three age groups had declined at the end of each experimental period. Thus, the capacity for fat mobilization was associated with tolerance to fasting. Plasma insulin concentrations in the rats in the three age groups were dramatically reduced during fasting periods, although basal levels of insulin were higher in the order of 32>8>3 week-old rats. Conclusion These results suggest that differences in fat metabolism patterns among rats in the three age groups during prolonged fasting were partly reflected the metabolic turnover rates, plasma insulin levels, and amounts of fat storage.

    Original languageEnglish
    Pages (from-to)157-163
    Number of pages7
    JournalEnvironmental Health and Preventive Medicine
    Volume17
    Issue number2
    DOIs
    Publication statusPublished - 2012 Mar

    Fingerprint

    Ketone Bodies
    Lipolysis
    Fasting
    Hydroxybutyrates
    Age Groups
    Nonesterified Fatty Acids
    Insulin
    Fats
    Weights and Measures
    White Adipose Tissue
    Sprague Dawley Rats
    Adipose Tissue

    Keywords

    • β-hydroxybutyrate, and acetoacetate
    • Aging
    • Fasting
    • Ketone body production
    • Lipolysis

    ASJC Scopus subject areas

    • Public Health, Environmental and Occupational Health

    Cite this

    Higashino-Matsui, Y., Shirato, K., Suzuki, Y., Kawashima, Y., Someya, Y., Sato, S., ... Imaizumi, K. (2012). Age-related effects of fasting on ketone body production during lipolysis in rats. Environmental Health and Preventive Medicine, 17(2), 157-163. https://doi.org/10.1007/s12199-011-0231-0

    Age-related effects of fasting on ketone body production during lipolysis in rats. / Higashino-Matsui, Yuriko; Shirato, Ken; Suzuki, Yuko; Kawashima, Yu; Someya, Yui; Sato, Shogo; Shiraishi, Akira; Jinde, Manabu; Matsumoto, Akiko; Ideno, Hisashi; Tachiyashiki, Kaoru; Imaizumi, Kazuhiko.

    In: Environmental Health and Preventive Medicine, Vol. 17, No. 2, 03.2012, p. 157-163.

    Research output: Contribution to journalArticle

    Higashino-Matsui, Y, Shirato, K, Suzuki, Y, Kawashima, Y, Someya, Y, Sato, S, Shiraishi, A, Jinde, M, Matsumoto, A, Ideno, H, Tachiyashiki, K & Imaizumi, K 2012, 'Age-related effects of fasting on ketone body production during lipolysis in rats', Environmental Health and Preventive Medicine, vol. 17, no. 2, pp. 157-163. https://doi.org/10.1007/s12199-011-0231-0
    Higashino-Matsui, Yuriko ; Shirato, Ken ; Suzuki, Yuko ; Kawashima, Yu ; Someya, Yui ; Sato, Shogo ; Shiraishi, Akira ; Jinde, Manabu ; Matsumoto, Akiko ; Ideno, Hisashi ; Tachiyashiki, Kaoru ; Imaizumi, Kazuhiko. / Age-related effects of fasting on ketone body production during lipolysis in rats. In: Environmental Health and Preventive Medicine. 2012 ; Vol. 17, No. 2. pp. 157-163.
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    abstract = "Objective The age-related effects of fasting on lipolysis, the production of ketone bodies, and plasma insulin levels were studied in male 3-, 8-, and 32-week-old Sprague- Dawley rats. Methods The rats were divided into fasting and control groups. The 3-, 8- and 32-week-old rats tolerated fasting for 2, 5, and 12 days, respectively. Results Fasting markedly reduced the weights of perirenal and periepididymal white adipose tissues in rats in the three age groups. The mean rates of reduction in both these adipose tissue weights during fasting periods were higher in the order of 3>8>32-week-old rats. Fasting transiently increased plasma free fatty acid (FFA), total ketone body, β-hydroxybutyrate, and acetoacetate concentrations in the rats in the three age groups. However, plasma FFA, total ketone body, β-hydroxybutyrate, and acetoacetate concentrations in the 3-week-old rats reached maximal peak within 2 days after the onset of fasting, although these concentrations in the 8- and 32-week-old rats took more than 2 days to reach the maximal peak. By contrast, the augmentation of plasma FFA, total ketone body, β-hydroxybutyrate, and acetoacetate concentrations in the rats in the three age groups had declined at the end of each experimental period. Thus, the capacity for fat mobilization was associated with tolerance to fasting. Plasma insulin concentrations in the rats in the three age groups were dramatically reduced during fasting periods, although basal levels of insulin were higher in the order of 32>8>3 week-old rats. Conclusion These results suggest that differences in fat metabolism patterns among rats in the three age groups during prolonged fasting were partly reflected the metabolic turnover rates, plasma insulin levels, and amounts of fat storage.",
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    AU - Higashino-Matsui, Yuriko

    AU - Shirato, Ken

    AU - Suzuki, Yuko

    AU - Kawashima, Yu

    AU - Someya, Yui

    AU - Sato, Shogo

    AU - Shiraishi, Akira

    AU - Jinde, Manabu

    AU - Matsumoto, Akiko

    AU - Ideno, Hisashi

    AU - Tachiyashiki, Kaoru

    AU - Imaizumi, Kazuhiko

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    N2 - Objective The age-related effects of fasting on lipolysis, the production of ketone bodies, and plasma insulin levels were studied in male 3-, 8-, and 32-week-old Sprague- Dawley rats. Methods The rats were divided into fasting and control groups. The 3-, 8- and 32-week-old rats tolerated fasting for 2, 5, and 12 days, respectively. Results Fasting markedly reduced the weights of perirenal and periepididymal white adipose tissues in rats in the three age groups. The mean rates of reduction in both these adipose tissue weights during fasting periods were higher in the order of 3>8>32-week-old rats. Fasting transiently increased plasma free fatty acid (FFA), total ketone body, β-hydroxybutyrate, and acetoacetate concentrations in the rats in the three age groups. However, plasma FFA, total ketone body, β-hydroxybutyrate, and acetoacetate concentrations in the 3-week-old rats reached maximal peak within 2 days after the onset of fasting, although these concentrations in the 8- and 32-week-old rats took more than 2 days to reach the maximal peak. By contrast, the augmentation of plasma FFA, total ketone body, β-hydroxybutyrate, and acetoacetate concentrations in the rats in the three age groups had declined at the end of each experimental period. Thus, the capacity for fat mobilization was associated with tolerance to fasting. Plasma insulin concentrations in the rats in the three age groups were dramatically reduced during fasting periods, although basal levels of insulin were higher in the order of 32>8>3 week-old rats. Conclusion These results suggest that differences in fat metabolism patterns among rats in the three age groups during prolonged fasting were partly reflected the metabolic turnover rates, plasma insulin levels, and amounts of fat storage.

    AB - Objective The age-related effects of fasting on lipolysis, the production of ketone bodies, and plasma insulin levels were studied in male 3-, 8-, and 32-week-old Sprague- Dawley rats. Methods The rats were divided into fasting and control groups. The 3-, 8- and 32-week-old rats tolerated fasting for 2, 5, and 12 days, respectively. Results Fasting markedly reduced the weights of perirenal and periepididymal white adipose tissues in rats in the three age groups. The mean rates of reduction in both these adipose tissue weights during fasting periods were higher in the order of 3>8>32-week-old rats. Fasting transiently increased plasma free fatty acid (FFA), total ketone body, β-hydroxybutyrate, and acetoacetate concentrations in the rats in the three age groups. However, plasma FFA, total ketone body, β-hydroxybutyrate, and acetoacetate concentrations in the 3-week-old rats reached maximal peak within 2 days after the onset of fasting, although these concentrations in the 8- and 32-week-old rats took more than 2 days to reach the maximal peak. By contrast, the augmentation of plasma FFA, total ketone body, β-hydroxybutyrate, and acetoacetate concentrations in the rats in the three age groups had declined at the end of each experimental period. Thus, the capacity for fat mobilization was associated with tolerance to fasting. Plasma insulin concentrations in the rats in the three age groups were dramatically reduced during fasting periods, although basal levels of insulin were higher in the order of 32>8>3 week-old rats. Conclusion These results suggest that differences in fat metabolism patterns among rats in the three age groups during prolonged fasting were partly reflected the metabolic turnover rates, plasma insulin levels, and amounts of fat storage.

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    KW - Fasting

    KW - Ketone body production

    KW - Lipolysis

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