Age-related expression analysis of mouse liver nuclear protein binding to 3′-untranslated region of Period2 gene

Toshiyuki Hamada, Kazuko Miyakawa, Hiroko Kushige, Shigenobu Shibata, Sumiko Kurachi

    Research output: Contribution to journalArticle

    Abstract

    In mammals, both circadian rhythm and aging play important roles in regulating time-dependent homeostasis. We previously discovered an age-related increase element binding protein, hnRNP A3, which binds to the 3′-untranslated region (UTR) of blood coagulation factor IX (FIX). Here, we describe other members of this protein family, hnRNP C and hnRNP H, which bind to the 3′-UTR of the mouse circadian clock gene Period 2 (mPer2). RNA electrophoretic mobility shift assays using a <sup>32</sup>P-labeled Per2 RNA probe coupled with two-dimensional gel electrophoresis followed by MALDI-TOF/MS peptide mass fingerprint analysis was used to analyze these proteins. Western blotting suggested that the total expression of these proteins in mouse liver cell nuclei does not increase with age. Two-dimensional gel electrophoresis analysis of age-related protein expression showed that many isoforms of these proteins exist in the liver and that each protein exhibits a complex age-related expression pattern. These results suggest that many isoforms of proteins are regulated by different aging systems and that many age regulation systems function in the liver.

    Original languageEnglish
    Pages (from-to)349-357
    Number of pages9
    JournalJournal of Physiological Sciences
    Volume65
    Issue number4
    DOIs
    Publication statusPublished - 2015 Apr 7

    Fingerprint

    3' Untranslated Regions
    Nuclear Proteins
    Protein Binding
    Liver
    Electrophoresis, Gel, Two-Dimensional
    Heterogeneous-Nuclear Ribonucleoprotein Group F-H
    Genes
    Heterogeneous-Nuclear Ribonucleoprotein Group C
    Protein Isoforms
    Proteins
    Heterogeneous-Nuclear Ribonucleoproteins
    RNA Probes
    Circadian Clocks
    Factor IX
    Peptide Mapping
    Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
    Electrophoretic Mobility Shift Assay
    Circadian Rhythm
    Cell Nucleus
    Mammals

    Keywords

    • Aging
    • Circadian rhythm
    • hnRNP
    • Homeostasis
    • Period 2

    ASJC Scopus subject areas

    • Physiology

    Cite this

    Age-related expression analysis of mouse liver nuclear protein binding to 3′-untranslated region of Period2 gene. / Hamada, Toshiyuki; Miyakawa, Kazuko; Kushige, Hiroko; Shibata, Shigenobu; Kurachi, Sumiko.

    In: Journal of Physiological Sciences, Vol. 65, No. 4, 07.04.2015, p. 349-357.

    Research output: Contribution to journalArticle

    Hamada, Toshiyuki ; Miyakawa, Kazuko ; Kushige, Hiroko ; Shibata, Shigenobu ; Kurachi, Sumiko. / Age-related expression analysis of mouse liver nuclear protein binding to 3′-untranslated region of Period2 gene. In: Journal of Physiological Sciences. 2015 ; Vol. 65, No. 4. pp. 349-357.
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