Aging characteristics of protein precipitates produced by polyelectrolyte precipitation in turbulently agitated reactor

Woon Soo Kim, Izumi Hirasawa, Woo Sik Kim

    Research output: Contribution to journalArticle

    8 Citations (Scopus)

    Abstract

    The relationship between aging and aggregation mechanism of solid lysozyme precipitates produced by polyacrylic acid (PAA) with a high molecular weight of 4.0 × 106 was experimentally investigated in a semi-batch reactor. As feeding the PAA, the aggregation mechanism between lysozyme and PAA was found to be determined by the aging history, which was shifted from charge neutralization to polymer bridging with increasing the agitation level. The lysozyme precipitates generated through a high bridging effect of PAA at a high mixing speed were broken up with aging, i.e. additional agitation of the product suspension after the feeding time. In contrast, the particle size of the precipitates formed at a low mixing intensity through a poor polymer bridging increased due to aging-induced aggregation, except for the extremely long aging time. Based on the largest particle size of the precipitates after aging, the aggregate strength was clearly confirmed to be enhanced by the bridging effect. Regardless of the mixing level, the lysozyme precipitates exhibited a steady particle size when the Camp number approached about 5 × 105 by adjusting the aging time with the same agitation speed as that used during the PAA feeding. However, the steady particle size was not observed when the Camp number was controlled by the aging intensity for the fixed aging time. The precipitates underwent the phenomenon of infilling at a high aging intensity, which appeared to participate, along with the shear stress, in the particle fragmentation that occurred during the aging process.

    Original languageEnglish
    Pages (from-to)4077-4085
    Number of pages9
    JournalChemical Engineering Science
    Volume57
    Issue number19
    DOIs
    Publication statusPublished - 2002 Oct 21

    Fingerprint

    carbopol 940
    Polyelectrolytes
    Precipitates
    Aging of materials
    Proteins
    Muramidase
    Enzymes
    Particle size
    Acids
    Agglomeration
    Polymers
    Batch reactors
    Shear stress
    Suspensions
    Molecular weight

    Keywords

    • Aggregation mechanism
    • Aging
    • Camp number
    • Lysozyme
    • Protein precipitation

    ASJC Scopus subject areas

    • Chemical Engineering(all)

    Cite this

    Aging characteristics of protein precipitates produced by polyelectrolyte precipitation in turbulently agitated reactor. / Kim, Woon Soo; Hirasawa, Izumi; Kim, Woo Sik.

    In: Chemical Engineering Science, Vol. 57, No. 19, 21.10.2002, p. 4077-4085.

    Research output: Contribution to journalArticle

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