Alcohol vapour detection at the three phase interface using enzyme-conducting polymer composites

Orawan Winther-Jensen, Robert Kerr, Bjorn Winther Jensen

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Immobilisation of enzymes on a breathable electrode can be useful for various applications where the three-phase interface between gas or chemical vapour, electrolyte and electrode is crucial for the reaction. In this paper, we report the further development of the breathable electrode concept by immobilisation of alcohol dehydrogenase into vapour-phase polymerised poly(3,4-ethylene dioxythiophene) that has been coated onto a breathable membrane. Typical alcohol sensing, whereby the coenzyme β-Nicotinamide adenine dinucleotide (NADH) is employed as a redox-mediator, was successfully used as a model reaction for the oxidation of ethanol. This indicates that the ethanol vapour from the backside of the membrane has access to the active enzyme embedded in the electrode. The detecting range of the sensor is suitable for the detection of ethanol in fruit juices and for the baseline breath ethanol concentration of drunken driving. After continuous operation for 4.5. h the system only showed a 20% decrease in the current output. The electrodes maintained 62% in current output after being refrigerated for 76 days. This work is continuing the progress of the immobilisation of specific enzymes for certain electrochemical reactions whereby the three-phase interface has to be maintained and/or the simultaneous separation of gas from liquid is required.

Original languageEnglish
Pages (from-to)143-146
Number of pages4
JournalBiosensors and Bioelectronics
Volume52
DOIs
Publication statusPublished - 2014 Feb 15
Externally publishedYes

Fingerprint

Phase interfaces
Conducting polymers
Polymers
Electrodes
Alcohols
Enzymes
Vapors
Ethanol
Immobilization
Composite materials
NAD
Gases
Fruit juices
Coenzymes
Membranes
Alcohol Dehydrogenase
Electrolytes
Oxidation-Reduction
Ethylene
Oxidation

Keywords

  • Biological materials
  • Breathable electrode
  • Gas separation
  • PEDOT
  • Stuffing method

ASJC Scopus subject areas

  • Biophysics
  • Biomedical Engineering
  • Biotechnology
  • Electrochemistry

Cite this

Alcohol vapour detection at the three phase interface using enzyme-conducting polymer composites. / Winther-Jensen, Orawan; Kerr, Robert; Winther Jensen, Bjorn.

In: Biosensors and Bioelectronics, Vol. 52, 15.02.2014, p. 143-146.

Research output: Contribution to journalArticle

Winther-Jensen, Orawan ; Kerr, Robert ; Winther Jensen, Bjorn. / Alcohol vapour detection at the three phase interface using enzyme-conducting polymer composites. In: Biosensors and Bioelectronics. 2014 ; Vol. 52. pp. 143-146.
@article{2c60c96d56b24b34b4797a6e5a4c1853,
title = "Alcohol vapour detection at the three phase interface using enzyme-conducting polymer composites",
abstract = "Immobilisation of enzymes on a breathable electrode can be useful for various applications where the three-phase interface between gas or chemical vapour, electrolyte and electrode is crucial for the reaction. In this paper, we report the further development of the breathable electrode concept by immobilisation of alcohol dehydrogenase into vapour-phase polymerised poly(3,4-ethylene dioxythiophene) that has been coated onto a breathable membrane. Typical alcohol sensing, whereby the coenzyme β-Nicotinamide adenine dinucleotide (NADH) is employed as a redox-mediator, was successfully used as a model reaction for the oxidation of ethanol. This indicates that the ethanol vapour from the backside of the membrane has access to the active enzyme embedded in the electrode. The detecting range of the sensor is suitable for the detection of ethanol in fruit juices and for the baseline breath ethanol concentration of drunken driving. After continuous operation for 4.5. h the system only showed a 20{\%} decrease in the current output. The electrodes maintained 62{\%} in current output after being refrigerated for 76 days. This work is continuing the progress of the immobilisation of specific enzymes for certain electrochemical reactions whereby the three-phase interface has to be maintained and/or the simultaneous separation of gas from liquid is required.",
keywords = "Biological materials, Breathable electrode, Gas separation, PEDOT, Stuffing method",
author = "Orawan Winther-Jensen and Robert Kerr and {Winther Jensen}, Bjorn",
year = "2014",
month = "2",
day = "15",
doi = "10.1016/j.bios.2013.08.033",
language = "English",
volume = "52",
pages = "143--146",
journal = "Biosensors",
issn = "0956-5663",
publisher = "Elsevier Limited",

}

TY - JOUR

T1 - Alcohol vapour detection at the three phase interface using enzyme-conducting polymer composites

AU - Winther-Jensen, Orawan

AU - Kerr, Robert

AU - Winther Jensen, Bjorn

PY - 2014/2/15

Y1 - 2014/2/15

N2 - Immobilisation of enzymes on a breathable electrode can be useful for various applications where the three-phase interface between gas or chemical vapour, electrolyte and electrode is crucial for the reaction. In this paper, we report the further development of the breathable electrode concept by immobilisation of alcohol dehydrogenase into vapour-phase polymerised poly(3,4-ethylene dioxythiophene) that has been coated onto a breathable membrane. Typical alcohol sensing, whereby the coenzyme β-Nicotinamide adenine dinucleotide (NADH) is employed as a redox-mediator, was successfully used as a model reaction for the oxidation of ethanol. This indicates that the ethanol vapour from the backside of the membrane has access to the active enzyme embedded in the electrode. The detecting range of the sensor is suitable for the detection of ethanol in fruit juices and for the baseline breath ethanol concentration of drunken driving. After continuous operation for 4.5. h the system only showed a 20% decrease in the current output. The electrodes maintained 62% in current output after being refrigerated for 76 days. This work is continuing the progress of the immobilisation of specific enzymes for certain electrochemical reactions whereby the three-phase interface has to be maintained and/or the simultaneous separation of gas from liquid is required.

AB - Immobilisation of enzymes on a breathable electrode can be useful for various applications where the three-phase interface between gas or chemical vapour, electrolyte and electrode is crucial for the reaction. In this paper, we report the further development of the breathable electrode concept by immobilisation of alcohol dehydrogenase into vapour-phase polymerised poly(3,4-ethylene dioxythiophene) that has been coated onto a breathable membrane. Typical alcohol sensing, whereby the coenzyme β-Nicotinamide adenine dinucleotide (NADH) is employed as a redox-mediator, was successfully used as a model reaction for the oxidation of ethanol. This indicates that the ethanol vapour from the backside of the membrane has access to the active enzyme embedded in the electrode. The detecting range of the sensor is suitable for the detection of ethanol in fruit juices and for the baseline breath ethanol concentration of drunken driving. After continuous operation for 4.5. h the system only showed a 20% decrease in the current output. The electrodes maintained 62% in current output after being refrigerated for 76 days. This work is continuing the progress of the immobilisation of specific enzymes for certain electrochemical reactions whereby the three-phase interface has to be maintained and/or the simultaneous separation of gas from liquid is required.

KW - Biological materials

KW - Breathable electrode

KW - Gas separation

KW - PEDOT

KW - Stuffing method

UR - http://www.scopus.com/inward/record.url?scp=84884229861&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84884229861&partnerID=8YFLogxK

U2 - 10.1016/j.bios.2013.08.033

DO - 10.1016/j.bios.2013.08.033

M3 - Article

C2 - 24055930

AN - SCOPUS:84884229861

VL - 52

SP - 143

EP - 146

JO - Biosensors

JF - Biosensors

SN - 0956-5663

ER -