Aldosterone induces circadian gene expression of clock genes in H9c2 cardiomyoblasts

Kyoe Tanaka, Naoto Ashizawa, Hiroaki Kawano, Osami Sato, Shinji Seto, Eijun Nishihara, Hideyuki Terazono, Shojiro Isomoto, Kazuyuki Shinohara, Katsusuke Yano

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

We examined mRNA expression of the clock genes (Per1, Per2, and Bmal1) and PAI-1 (plasminogen activator inhibitor-1) after aldosterone treatment every 4h up to 48h in H9c2 cardiomyoblasts by reverse transcription-polymerase chain reaction. To block the MR (mineralocorticoid receptor), the MR antagonist, spironolactone, was added to the medium 1h before aldosterone treatment. Aldosterone induced an initial increase and rhythmic expression of Per1, while spironolactone attenuated the acute increase in Per1 mRNA induced by aldosterone. On the other hand, aldosterone did not increase the Per2 mRNA in the acute phase, but thereafter induced a rhythmic expression of Per2. Aldosterone also induced rhythmic expression of Bmal1, a positive element of the clock genes. The rhythm of Bmal1 mRNA was anti-phase of that of Per2 mRNA. Aldosterone induced an acute increase in PAI-1 mRNA, but did not induce rhythmic expression of PAI-1. The present study demonstrated first that aldosterone regulates expression of the clock genes Per1, Per2, and Bmal1, and increases PAI-1 expression in H9c2 cardiomyoblasts. Second, an acute increase in Per1 mRNA after aldosterone treatment is mediated through MR. Third, clock genes are not related to PAI-1 expression in H9c2 cardiomyoblasts.

Original languageEnglish
Pages (from-to)254-260
Number of pages7
JournalHeart and Vessels
Volume22
Issue number4
DOIs
Publication statusPublished - 2007 Jul
Externally publishedYes

    Fingerprint

Keywords

  • Aldosterone
  • Cardiomyoblast
  • Clock gene
  • PAI-1

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

Tanaka, K., Ashizawa, N., Kawano, H., Sato, O., Seto, S., Nishihara, E., Terazono, H., Isomoto, S., Shinohara, K., & Yano, K. (2007). Aldosterone induces circadian gene expression of clock genes in H9c2 cardiomyoblasts. Heart and Vessels, 22(4), 254-260. https://doi.org/10.1007/s00380-006-0968-3