Allyl isothiocyanate-induced changes in the distribution of white blood cells in rats

Kazuhiko Imaizumi, Shogo Sato, Yuko Sakakibara, Sayuri Mori, Masaki Ohkuma, Yu Kawashima, Takamasa Ban, Hiromi Sasaki, Kaoru Tachiyashiki

    Research output: Contribution to journalArticle

    11 Citations (Scopus)

    Abstract

    The main pungent component of wasabi (Eutrema japonica) is known to be isothiocyanate and its derivatives, volatile substances. Allyl isothiocyanate (AITC) accounts for more than half of isothiocyanate derivatives. However, there is a little information on the effects of AITC on the immune system by analyzing the number of white blood cells (WBCs) over the course of days of AITC administration. In the present study, we studied the effects of AITC (dose=20 mg/kg body weight/day for 10 days, subcutaneous: s.c.) on the number of WBCs (total WBCs, lymphocytes, monocyte, neutrophil, basophil and eosinophil) and plasma corticosterone concentrations in adult male rats. Administration of AITC decreased significantly the number of total WBCs on days 1-4 post s.c. injection by 25-27%. Administration of AITC also decreased the number of lymphocytes on days 1-10 by 21-36% and monocyte on days 1-8 by 28-78%. However, administration of AITC increased the number of neutrophil on days 8-10 by 61-112%. AITC did not change the number of eosinophil and basophil. Plasma corticosterone concentrations during the experimental period were 4.7-8.4 times significantly higher in the AITC group than in the control group, indicating that AITC induced stress-responses. The relative weights of thymus and adrenals per body weight were significantly lower and clearly higher in the AITC group than in the control group, respectively. These results suggest that AITC-mediated stress-responses are at least in part attributable to changes in the number of circulating WBCs.

    Original languageEnglish
    Pages (from-to)583-589
    Number of pages7
    JournalJournal of Toxicological Sciences
    Volume35
    Issue number4
    DOIs
    Publication statusPublished - 2010 Aug

    Fingerprint

    Rats
    Leukocytes
    Blood
    Cells
    Lymphocytes
    Basophils
    Corticosterone
    Eosinophils
    allyl isothiocyanate
    Monocytes
    Neutrophils
    Body Weight
    Derivatives
    Plasmas
    Thymus
    Control Groups
    Immune system
    Lymphocyte Count
    Thymus Gland
    Immune System

    Keywords

    • Allyl isothiocyanate
    • Lymphocytes
    • Plasma corticosterone
    • Wasabi
    • White blood cells

    ASJC Scopus subject areas

    • Toxicology

    Cite this

    Imaizumi, K., Sato, S., Sakakibara, Y., Mori, S., Ohkuma, M., Kawashima, Y., ... Tachiyashiki, K. (2010). Allyl isothiocyanate-induced changes in the distribution of white blood cells in rats. Journal of Toxicological Sciences, 35(4), 583-589. https://doi.org/10.2131/jts.35.583

    Allyl isothiocyanate-induced changes in the distribution of white blood cells in rats. / Imaizumi, Kazuhiko; Sato, Shogo; Sakakibara, Yuko; Mori, Sayuri; Ohkuma, Masaki; Kawashima, Yu; Ban, Takamasa; Sasaki, Hiromi; Tachiyashiki, Kaoru.

    In: Journal of Toxicological Sciences, Vol. 35, No. 4, 08.2010, p. 583-589.

    Research output: Contribution to journalArticle

    Imaizumi, K, Sato, S, Sakakibara, Y, Mori, S, Ohkuma, M, Kawashima, Y, Ban, T, Sasaki, H & Tachiyashiki, K 2010, 'Allyl isothiocyanate-induced changes in the distribution of white blood cells in rats', Journal of Toxicological Sciences, vol. 35, no. 4, pp. 583-589. https://doi.org/10.2131/jts.35.583
    Imaizumi, Kazuhiko ; Sato, Shogo ; Sakakibara, Yuko ; Mori, Sayuri ; Ohkuma, Masaki ; Kawashima, Yu ; Ban, Takamasa ; Sasaki, Hiromi ; Tachiyashiki, Kaoru. / Allyl isothiocyanate-induced changes in the distribution of white blood cells in rats. In: Journal of Toxicological Sciences. 2010 ; Vol. 35, No. 4. pp. 583-589.
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