Alteration of basal release of anterior pituitary hormones by pretreatment of primary cultured cells with trypsin

Shiota Kunio, Kasuga Fumiko, Takamori Shigeo, Ogawa Tomoya

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The anterior pituitary (AP) gland secretes 6 different hormones. Prolactin (PRL) is secreted at a relatively high level without stimulation by the hypothalamus, while secretion of the others requires the action of stimulatory factors from the hypothalamus. In order to gain an insight into the mechanism underlying the different spontaneous release patterns of these hormones, we investigated their spontaneous release rate after pretreating rat anterior pituitary cells with trypsin. Rat AP cells were cultured on Cytodex microcarrier beads for 4 days and were then superfused with either control medium or medium containing trypsin (0.25%) for 5 min. The subsequent release rates of the AP hormones were monitored. The basal release of PRL was severely reduced to almost undetectable level and began to recover 120 min after the trypsin-pretreatment. Full recovery was attained over the next 100 min and was delayed by treatment with a protein synthesis inhibitor, cycloheximide (7 μM). In the trypsin-pretreated cells, basal release of PRL and growth hormone (GH) was severely reduced, while that of thyroid stimulating hormone (TSH) and adrenocorticotropic hormone (ACTH) was enhanced and luteinizing hormone (LH) and follicle stimulating hormone (FSH) was not markedly affected by the treatment, suggesting that the suppression of PRL release was not caused by nonspecific damage to the cells. Since trypsin does not readily enter cells, the altered secretion of AP hormones seems to be the result of restricted digestion of the external components of the cells. On the bases of these observations, we predicted that the mechanism of spontaneous release of hormones involves trypsin sensitive proteins (TSMP) on the plasma membranes of the anterior pituitary cells.

Original languageEnglish
Pages (from-to)315-320
Number of pages6
JournalMolecular and Cellular Endocrinology
Volume101
Issue number1-2
DOIs
Publication statusPublished - 1994
Externally publishedYes

Fingerprint

Anterior Pituitary Hormones
Trypsin
Cultured Cells
Cells
Prolactin
Hormones
Hypothalamus
Rats
Protein Synthesis Inhibitors
Anterior Pituitary Gland
Follicle Stimulating Hormone
Thyrotropin
Cellular Structures
Cell membranes
Cycloheximide
Luteinizing Hormone
Adrenocorticotropic Hormone
Growth Hormone
Digestion
Cell Membrane

Keywords

  • Anterior pituitary cells
  • Hormone release
  • Prolactin
  • Trypsin sensitive protein

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Alteration of basal release of anterior pituitary hormones by pretreatment of primary cultured cells with trypsin. / Kunio, Shiota; Fumiko, Kasuga; Shigeo, Takamori; Tomoya, Ogawa.

In: Molecular and Cellular Endocrinology, Vol. 101, No. 1-2, 1994, p. 315-320.

Research output: Contribution to journalArticle

Kunio, Shiota ; Fumiko, Kasuga ; Shigeo, Takamori ; Tomoya, Ogawa. / Alteration of basal release of anterior pituitary hormones by pretreatment of primary cultured cells with trypsin. In: Molecular and Cellular Endocrinology. 1994 ; Vol. 101, No. 1-2. pp. 315-320.
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