Alterations in RFamide-related peptide expression are coordinated with the preovulatory luteinizing hormone surge

Erin M. Gibson, Stephanie A. Humber, Sachi Jain, Wilbur P. Williams, Sheng Zhao, George E. Bentley, Kazuyoshi Tsutsui, Lance J. Kriegsfeld

    Research output: Contribution to journalArticle

    147 Citations (Scopus)

    Abstract

    The preovulatory LH surge is triggered when the circadian pacemaker, the bilateral suprachiasmatic nucleus (SCN), stimulates the GnRH system in the presence of high estrogen concentrations (positive feedback). Importantly, during the remainder of the estrous cycle, estradiol inhibits LH release via negative feedback. We have recently documented the presence of a novel mammalian RFamide-related peptide (RFRP), a putative gonadotropin-inhibitory hormone (GnIH), that presumably acts upstream of GnRH to modulate the negative feedback effects of estrogen. The present series of studies used female Syrian hamsters to examine the possibility that, in addition to driving the LH surge positively, the SCN concomitantly coordinates the removal of steroid-mediated RFRP inhibition of the gonadotropic axis to permit the surge. We found that the SCN forms close appositions with RFRP cells, suggesting the possibility for direct temporal control of RFRP activity. During the time of the LH surge, immediate-early gene expression is reduced in RFRP cells, and this temporal regulation is estrogen dependent. To determine whether projections from the SCN regulate the timed reduction in activation of the RFRP system, we exploited the phenomenon of splitting. In split animals in which the SCN are active in antiphase, activation of the RFRP system is asymmetrical. Importantly, this asymmetry is opposite to the state of the GnRH system. Together, these findings point to novel circadian control of the RFRP system and potential participation in the circuitry controlling ovulatory function.

    Original languageEnglish
    Pages (from-to)4958-4969
    Number of pages12
    JournalEndocrinology
    Volume149
    Issue number10
    DOIs
    Publication statusPublished - 2008 Oct

    Fingerprint

    Luteinizing Hormone
    Suprachiasmatic Nucleus
    Gonadotropin-Releasing Hormone
    Estrogens
    Immediate-Early Genes
    Estrous Cycle
    RFamide peptide
    Mesocricetus
    Gonadotropins
    Estradiol
    Steroids
    Hormones
    Gene Expression

    ASJC Scopus subject areas

    • Endocrinology

    Cite this

    Gibson, E. M., Humber, S. A., Jain, S., Williams, W. P., Zhao, S., Bentley, G. E., ... Kriegsfeld, L. J. (2008). Alterations in RFamide-related peptide expression are coordinated with the preovulatory luteinizing hormone surge. Endocrinology, 149(10), 4958-4969. https://doi.org/10.1210/en.2008-0316

    Alterations in RFamide-related peptide expression are coordinated with the preovulatory luteinizing hormone surge. / Gibson, Erin M.; Humber, Stephanie A.; Jain, Sachi; Williams, Wilbur P.; Zhao, Sheng; Bentley, George E.; Tsutsui, Kazuyoshi; Kriegsfeld, Lance J.

    In: Endocrinology, Vol. 149, No. 10, 10.2008, p. 4958-4969.

    Research output: Contribution to journalArticle

    Gibson, EM, Humber, SA, Jain, S, Williams, WP, Zhao, S, Bentley, GE, Tsutsui, K & Kriegsfeld, LJ 2008, 'Alterations in RFamide-related peptide expression are coordinated with the preovulatory luteinizing hormone surge', Endocrinology, vol. 149, no. 10, pp. 4958-4969. https://doi.org/10.1210/en.2008-0316
    Gibson, Erin M. ; Humber, Stephanie A. ; Jain, Sachi ; Williams, Wilbur P. ; Zhao, Sheng ; Bentley, George E. ; Tsutsui, Kazuyoshi ; Kriegsfeld, Lance J. / Alterations in RFamide-related peptide expression are coordinated with the preovulatory luteinizing hormone surge. In: Endocrinology. 2008 ; Vol. 149, No. 10. pp. 4958-4969.
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