Altered subcellular localization of a tobacco membrane raft-associated remorin protein by tobamovirus infection and transient expression of viral replication and movement proteins

Nobumitsu Sasaki, Eita Takashima, Hiroshi Nyunoya

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1 Citation (Scopus)

Abstract

Remorins are plant specific proteins found in plasma membrane microdomains (termed lipid or membrane rafts) and plasmodesmata. A potato remorin is reported to be involved in negatively regulating potexvirus movement and plasmodesmal permeability. In this study, we isolated cDNAs of tobacco remorins (NtREMs) and examined roles of an NtREM in infection by tomato mosaic virus (ToMV). Subcellular localization analysis using fluorescently tagged NtREM, ToMV, and viral replication and movement proteins (MPs) indicated that virus infection and transient expression of the viral proteins promoted the formation of NtREM aggregates by altering the subcellular distribution of NtREM, which was localized uniformly on the plasma membrane under normal conditions. NtREM aggregates were often observed associated closely with endoplasmic reticulum networks and bodies of the 126K replication and MPs. The bimolecular fluorescence complementation assay indicated that NtREM might interact directly with the MP on the plasma membrane and around plasmodesmata. In addition, transient overexpression of NtREM facilitated ToMV cell-to-cell movement. Based on these results, we discuss possible roles of the tobacco remorin in tobamovirus movement.

Original languageEnglish
Article number619
JournalFrontiers in Plant Science
Volume9
DOIs
Publication statusPublished - 2018 May 15

Fingerprint

Tobamovirus
Tomato mosaic virus
virus replication
tobacco
plasmodesmata
plasma membrane
protein aggregates
infection
proteins
Potexvirus
viral proteins
cell movement
endoplasmic reticulum
permeability
potatoes
fluorescence
viruses
assays
lipids
cells

Keywords

  • BiFC
  • Cell-to-cell movement
  • Membrane raft
  • Nicotiana benthamiana
  • Plasma membrane
  • Remorin
  • Tobacco
  • Tomato mosaic virus

ASJC Scopus subject areas

  • Plant Science

Cite this

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title = "Altered subcellular localization of a tobacco membrane raft-associated remorin protein by tobamovirus infection and transient expression of viral replication and movement proteins",
abstract = "Remorins are plant specific proteins found in plasma membrane microdomains (termed lipid or membrane rafts) and plasmodesmata. A potato remorin is reported to be involved in negatively regulating potexvirus movement and plasmodesmal permeability. In this study, we isolated cDNAs of tobacco remorins (NtREMs) and examined roles of an NtREM in infection by tomato mosaic virus (ToMV). Subcellular localization analysis using fluorescently tagged NtREM, ToMV, and viral replication and movement proteins (MPs) indicated that virus infection and transient expression of the viral proteins promoted the formation of NtREM aggregates by altering the subcellular distribution of NtREM, which was localized uniformly on the plasma membrane under normal conditions. NtREM aggregates were often observed associated closely with endoplasmic reticulum networks and bodies of the 126K replication and MPs. The bimolecular fluorescence complementation assay indicated that NtREM might interact directly with the MP on the plasma membrane and around plasmodesmata. In addition, transient overexpression of NtREM facilitated ToMV cell-to-cell movement. Based on these results, we discuss possible roles of the tobacco remorin in tobamovirus movement.",
keywords = "BiFC, Cell-to-cell movement, Membrane raft, Nicotiana benthamiana, Plasma membrane, Remorin, Tobacco, Tomato mosaic virus",
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T1 - Altered subcellular localization of a tobacco membrane raft-associated remorin protein by tobamovirus infection and transient expression of viral replication and movement proteins

AU - Sasaki, Nobumitsu

AU - Takashima, Eita

AU - Nyunoya, Hiroshi

PY - 2018/5/15

Y1 - 2018/5/15

N2 - Remorins are plant specific proteins found in plasma membrane microdomains (termed lipid or membrane rafts) and plasmodesmata. A potato remorin is reported to be involved in negatively regulating potexvirus movement and plasmodesmal permeability. In this study, we isolated cDNAs of tobacco remorins (NtREMs) and examined roles of an NtREM in infection by tomato mosaic virus (ToMV). Subcellular localization analysis using fluorescently tagged NtREM, ToMV, and viral replication and movement proteins (MPs) indicated that virus infection and transient expression of the viral proteins promoted the formation of NtREM aggregates by altering the subcellular distribution of NtREM, which was localized uniformly on the plasma membrane under normal conditions. NtREM aggregates were often observed associated closely with endoplasmic reticulum networks and bodies of the 126K replication and MPs. The bimolecular fluorescence complementation assay indicated that NtREM might interact directly with the MP on the plasma membrane and around plasmodesmata. In addition, transient overexpression of NtREM facilitated ToMV cell-to-cell movement. Based on these results, we discuss possible roles of the tobacco remorin in tobamovirus movement.

AB - Remorins are plant specific proteins found in plasma membrane microdomains (termed lipid or membrane rafts) and plasmodesmata. A potato remorin is reported to be involved in negatively regulating potexvirus movement and plasmodesmal permeability. In this study, we isolated cDNAs of tobacco remorins (NtREMs) and examined roles of an NtREM in infection by tomato mosaic virus (ToMV). Subcellular localization analysis using fluorescently tagged NtREM, ToMV, and viral replication and movement proteins (MPs) indicated that virus infection and transient expression of the viral proteins promoted the formation of NtREM aggregates by altering the subcellular distribution of NtREM, which was localized uniformly on the plasma membrane under normal conditions. NtREM aggregates were often observed associated closely with endoplasmic reticulum networks and bodies of the 126K replication and MPs. The bimolecular fluorescence complementation assay indicated that NtREM might interact directly with the MP on the plasma membrane and around plasmodesmata. In addition, transient overexpression of NtREM facilitated ToMV cell-to-cell movement. Based on these results, we discuss possible roles of the tobacco remorin in tobamovirus movement.

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KW - Cell-to-cell movement

KW - Membrane raft

KW - Nicotiana benthamiana

KW - Plasma membrane

KW - Remorin

KW - Tobacco

KW - Tomato mosaic virus

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