Alternately binding probe competitive PCR as a simple, cost-effective, and accurate quantification method for JAK2V617F allele burden in myeloproliferative neoplasms

Soji Morishita, Norio Komatsu, Keita Kirito, Aya H. Koda, Yuji Sekiguchi, Satoshi Tsuneda, Naohiro Noda

Research output: Contribution to journalArticle

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Abstract

We developed a simple, cost-effective, and accurate JAK2 allele burden quantification method named alternately binding probe competitive PCR (ABC-PCR). ABC-PCR can be performed to quantify target JAK2 allele burdens in a single reaction. The throughput and running cost of ABC-PCR are markedly improved compared with those of allele-specific quantitative PCR (AS-qPCR). The quantification of samples with known JAK2 allele burdens revealed that ABC-PCR had a small assay-to-assay variation. The JAK2 allele burdens in the patients with myeloproliferative neoplasms measured by ABC-PCR and AS-qPCR showed a good fitting. ABC-PCR would be a powerful tool for quantifying target JAK2 allele burdens.

Original languageEnglish
Pages (from-to)1632-1636
Number of pages5
JournalLeukemia Research
Volume35
Issue number12
DOIs
Publication statusPublished - 2011 Dec 1

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Keywords

  • Allele burden
  • Fluorescence quenching
  • Gene quantification
  • JAK2V617F mutation
  • Myeloproliferative neoplasms

ASJC Scopus subject areas

  • Hematology
  • Oncology
  • Cancer Research

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