Amino-silane modified superparamagnetic particles with surface-immobilized enzyme

Hideki Kobayashi, Tadashi Matsunaga

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

Microfine magnetic particles of magnetite with a mean diameter of 10-15 nm, which is in the range of superparamagnetism, were prepared by coprecipitation from ferrous and ferric electrolyte solution and applied to the enzyme immobilization support. The surface of the particles was treated by a series of amino-functional silane coupling agents capable of covalently bonding with enzyme. A protease, thermolysin, was immobilized on the particles and transferred to ethyl acetate solution containing N-Cbz-l-aspartic acid and l-phenylalanine methyl ester. The dipeptide, aspartame precursor synthetic reaction was then carried out. Higher enzyme activity was found when the silane coupling agents with long spacer arms between the silane functionality and amino functionality were used such as N-(6-aminohexyl)-3-aminopropyltrimethyoxysilane, compared to conventional 3-aminopropyltrichoxysilane. Magnetic recovery of the immobilized enzyme was also demonstrated upon the application of an external magnetic field.

Original languageEnglish
Pages (from-to)505-511
Number of pages7
JournalJournal of Colloid and Interface Science
Volume141
Issue number2
DOIs
Publication statusPublished - 1991
Externally publishedYes

Fingerprint

Silanes
Immobilized Enzymes
Enzymes
Coupling agents
Aspartame
Superparamagnetism
Ferrosoferric Oxide
Thermolysin
Enzyme immobilization
Dipeptides
Enzyme activity
Magnetite
Coprecipitation
Aspartic Acid
Electrolytes
Esters
Peptide Hydrolases
Magnetic fields
Recovery
Acids

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Surfaces, Coatings and Films
  • Colloid and Surface Chemistry

Cite this

Amino-silane modified superparamagnetic particles with surface-immobilized enzyme. / Kobayashi, Hideki; Matsunaga, Tadashi.

In: Journal of Colloid and Interface Science, Vol. 141, No. 2, 1991, p. 505-511.

Research output: Contribution to journalArticle

Kobayashi, Hideki ; Matsunaga, Tadashi. / Amino-silane modified superparamagnetic particles with surface-immobilized enzyme. In: Journal of Colloid and Interface Science. 1991 ; Vol. 141, No. 2. pp. 505-511.
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