Amperometric determination of viable cell numbers based on sensing microbial respiration

Tadashi Matsunaga, Isao Karube, Takashi Nakahara, Shuichi Suzuki

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13 Citations (Scopus)

Abstract

Viable cell numbers were determined with an electrode system composed of a membrane filter for retaining microorganisms and an oxygen electrode. The principle of the cell number determination is based on sensing microbial respiration. The response time of the system was 10 min. A linear relationship was obtained between the current and the cell numbers of Escherichia coli, Bacillus subtilis, Flavobacterium arabrescence, Saccharomyces cerevisiae, and Trichosporon cutaneum on the membrane filter. The current was reproducible, with an average relative error of 5% when the electrode contained 2×108Escherichia coli. A good agreement (a correlation coefficient; 0.98 in 10 experiments) was obtained, when the cell populations of E. coli were determined by the proposed method and colony counts. The minimum measurable cell populations were 106 cells per ml with the bacteria and 105 cells per ml with the yeasts.

Original languageEnglish
Pages (from-to)97-101
Number of pages5
JournalEuropean Journal of Applied Microbiology and Biotechnology
Volume12
Issue number2
DOIs
Publication statusPublished - 1981 Jun
Externally publishedYes

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ASJC Scopus subject areas

  • Microbiology
  • Biotechnology
  • Medicine(all)

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