Amyloid-β25-35 induces impairment of cognitive function and long-term potentiation through phosphorylation of collapsin response mediator protein 2

Toshinari Isono, Naoya Yamashita, Masami Obara, Tomomi Araki, Fumio Nakamura, Yoshinori Kamiya, Tursun Alkam, Atsumi Nitta, Toshitaka Nabeshima, Katsuhiko Mikoshiba, Toshio Ohshima, Yoshio Goshima

    Research output: Contribution to journalArticle

    23 Citations (Scopus)

    Abstract

    Alzheimer's disease (AD) is characterized by amyloid-β (Aβ) protein and tau deposition in the brain. Numerous studies have reported a central role of Aβ in the development of AD, but the pathogenesis is not well understood. Collapsin response mediator protein 2 (CRMP2), an intracellular protein mediating a repulsive axon guidance molecule, Semaphorin3A, is also accumulated in neurofibrillary tangles in AD brains. To gain insight into the role of CRMP2 phosphorylation in AD pathogenesis, we investigated the effects of Aβ neurotoxicity in CRMP2 phosphorylation-deficient knock-in (crmp2ki/ki) mice, in which the serine residue at 522 was replaced with alanine. Intracerebroventricular (i.c.v.) injection of Aβ25-35 peptide, a neurotoxic fragment of Aβ protein, to wild-type (wt) mice increased hippocampal phosphorylation of CRMP2. Behavioral assessment revealed that i.c.v. injection of Aβ25-35 peptide caused impairment of novel object recognition in wt mice, while the same peptide did not in crmp2ki/ki mice. In electrophysiological recording, wt and crmp2ki/ki mice have similar input-output basal synaptic transmission and paired-pulse ratios. However, long-term potentiation was impaired in hippocampal slices of Aβ25-35 peptide-treated wt but not those of crmp2ki/ki. Our findings indicate that CRMP2 phosphorylation is required for Aβ-induced impairment of cognitive memory and synaptic plasticity.

    Original languageEnglish
    Pages (from-to)180-185
    Number of pages6
    JournalNeuroscience Research
    Volume77
    Issue number3
    DOIs
    Publication statusPublished - 2013 Nov

    Fingerprint

    Long-Term Potentiation
    Amyloid
    Cognition
    Phosphorylation
    Alzheimer Disease
    Peptides
    Serum Amyloid A Protein
    Neurofibrillary Tangles
    Injections
    Neuronal Plasticity
    Brain
    Synaptic Transmission
    Alanine
    Serine
    Proteins
    collapsin response mediator protein-2

    Keywords

    • Alzheimer's disease
    • Cyclin-dependent kinase 5
    • Knock-in mice
    • Neurotoxicity
    • Novel object recognition
    • Phosphorylation

    ASJC Scopus subject areas

    • Neuroscience(all)

    Cite this

    Amyloid-β25-35 induces impairment of cognitive function and long-term potentiation through phosphorylation of collapsin response mediator protein 2. / Isono, Toshinari; Yamashita, Naoya; Obara, Masami; Araki, Tomomi; Nakamura, Fumio; Kamiya, Yoshinori; Alkam, Tursun; Nitta, Atsumi; Nabeshima, Toshitaka; Mikoshiba, Katsuhiko; Ohshima, Toshio; Goshima, Yoshio.

    In: Neuroscience Research, Vol. 77, No. 3, 11.2013, p. 180-185.

    Research output: Contribution to journalArticle

    Isono, T, Yamashita, N, Obara, M, Araki, T, Nakamura, F, Kamiya, Y, Alkam, T, Nitta, A, Nabeshima, T, Mikoshiba, K, Ohshima, T & Goshima, Y 2013, 'Amyloid-β25-35 induces impairment of cognitive function and long-term potentiation through phosphorylation of collapsin response mediator protein 2', Neuroscience Research, vol. 77, no. 3, pp. 180-185. https://doi.org/10.1016/j.neures.2013.08.005
    Isono, Toshinari ; Yamashita, Naoya ; Obara, Masami ; Araki, Tomomi ; Nakamura, Fumio ; Kamiya, Yoshinori ; Alkam, Tursun ; Nitta, Atsumi ; Nabeshima, Toshitaka ; Mikoshiba, Katsuhiko ; Ohshima, Toshio ; Goshima, Yoshio. / Amyloid-β25-35 induces impairment of cognitive function and long-term potentiation through phosphorylation of collapsin response mediator protein 2. In: Neuroscience Research. 2013 ; Vol. 77, No. 3. pp. 180-185.
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    AU - Araki, Tomomi

    AU - Nakamura, Fumio

    AU - Kamiya, Yoshinori

    AU - Alkam, Tursun

    AU - Nitta, Atsumi

    AU - Nabeshima, Toshitaka

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