An application of a two-dimensional photoncounter for the determination of emission spectra of thermoluminescence from photosynthetic systems

Kintake Sonoike, Yorinao Inoue

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Abstract

Emission spectra of two components of thermoluminescence from photosynthetic systems were measured using a two-dimensional photoncounter. Application of this detector system enormously improved the signal to noise (S/N) ratio and enabled the determination of an emission spectrum of a specific thermoluminescence component. The B-band emitting at 30°C showed an emission spectrum having a single peak at 690 nm, whereas the Z-band emitting at -160°C showed a spectrum having two peaks, the major band at 740 nm and a satellite band at 690 nm. It was revealed that the longer wavelength component arises from photosystem I and the shorter wavelength component from photosystem II. Light harvesting chlorophyll a/b protein complexes (LHCI and LHCII) were also found to emit the Z-band at 740 and 690 nm, respectively. The advantage of a two-dimensional photoncounter for the determination of emission spectra of weak luminescence from large-surface biological samples is discussed.

Original languageEnglish
Pages (from-to)129-137
Number of pages9
JournalJournal of Luminescence
Volume51
Issue number1-3
DOIs
Publication statusPublished - 1992 Feb

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ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Chemistry(all)
  • Atomic and Molecular Physics, and Optics
  • Condensed Matter Physics

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