TY - JOUR
T1 - An application of a two-dimensional photoncounter for the determination of emission spectra of thermoluminescence from photosynthetic systems
AU - Sonoike, Kintake
AU - Inoue, Yorinao
N1 - Funding Information:
The authors acknowledge the financial support by the following grants: a Special Coordination Fund for Development of Time-resolved Fluorescence Microscope and a Grant on Solar Energy Conversion by Means of Photosynthesis, both given by the Science and Technology Agency (STA) of Japan, and MESC Grants-in-Aid for Cooperative Research No. 0132064 and 0130009. K.S. is thankful to Dr. M. Taguchi for her assistance in preparing the manuscript.
PY - 1992/2
Y1 - 1992/2
N2 - Emission spectra of two components of thermoluminescence from photosynthetic systems were measured using a two-dimensional photoncounter. Application of this detector system enormously improved the signal to noise (S/N) ratio and enabled the determination of an emission spectrum of a specific thermoluminescence component. The B-band emitting at 30°C showed an emission spectrum having a single peak at 690 nm, whereas the Z-band emitting at -160°C showed a spectrum having two peaks, the major band at 740 nm and a satellite band at 690 nm. It was revealed that the longer wavelength component arises from photosystem I and the shorter wavelength component from photosystem II. Light harvesting chlorophyll a/b protein complexes (LHCI and LHCII) were also found to emit the Z-band at 740 and 690 nm, respectively. The advantage of a two-dimensional photoncounter for the determination of emission spectra of weak luminescence from large-surface biological samples is discussed.
AB - Emission spectra of two components of thermoluminescence from photosynthetic systems were measured using a two-dimensional photoncounter. Application of this detector system enormously improved the signal to noise (S/N) ratio and enabled the determination of an emission spectrum of a specific thermoluminescence component. The B-band emitting at 30°C showed an emission spectrum having a single peak at 690 nm, whereas the Z-band emitting at -160°C showed a spectrum having two peaks, the major band at 740 nm and a satellite band at 690 nm. It was revealed that the longer wavelength component arises from photosystem I and the shorter wavelength component from photosystem II. Light harvesting chlorophyll a/b protein complexes (LHCI and LHCII) were also found to emit the Z-band at 740 and 690 nm, respectively. The advantage of a two-dimensional photoncounter for the determination of emission spectra of weak luminescence from large-surface biological samples is discussed.
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U2 - 10.1016/0022-2313(92)90025-5
DO - 10.1016/0022-2313(92)90025-5
M3 - Article
AN - SCOPUS:0026821821
VL - 51
SP - 129
EP - 137
JO - Journal of Luminescence
JF - Journal of Luminescence
SN - 0022-2313
IS - 1-3
ER -