Abstract
Emission spectra of two components of thermoluminescence from photosynthetic systems were measured using a two-dimensional photoncounter. Application of this detector system enormously improved the signal to noise (S/N) ratio and enabled the determination of an emission spectrum of a specific thermoluminescence component. The B-band emitting at 30°C showed an emission spectrum having a single peak at 690 nm, whereas the Z-band emitting at -160°C showed a spectrum having two peaks, the major band at 740 nm and a satellite band at 690 nm. It was revealed that the longer wavelength component arises from photosystem I and the shorter wavelength component from photosystem II. Light harvesting chlorophyll a/b protein complexes (LHCI and LHCII) were also found to emit the Z-band at 740 and 690 nm, respectively. The advantage of a two-dimensional photoncounter for the determination of emission spectra of weak luminescence from large-surface biological samples is discussed.
Original language | English |
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Pages (from-to) | 129-137 |
Number of pages | 9 |
Journal | Journal of Luminescence |
Volume | 51 |
Issue number | 1-3 |
DOIs | |
Publication status | Published - 1992 Feb |
Externally published | Yes |
ASJC Scopus subject areas
- Biophysics
- Biochemistry
- Chemistry(all)
- Atomic and Molecular Physics, and Optics
- Condensed Matter Physics