An application of photoactivatable substrate for the evaluation of epithelial-mesenchymal transition inhibitors

Jun Nakanishi, Kenji Sugiyama, Hirotaka Matsuo, Yo Ko Takahashi, Satoshi O. Mura, Takuji Nakashima

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Epithelial-mesenchymal transition (EMT), phenotypic changes in cell adhesion and migration, is involved in cancer invasion and metastasis, hence becoming a target for anti-cancer drugs. In this study, we report a method for the evaluation of EMT inhibitors by using a photoactivatable gold substrate, which changes from non-cell-adhesive to celladhesive in response to light. The method is based on the geometrical confinement of cell clusters and the subsequent migration induction by controlled photoirradiation of the substrate. As a proof-of-concept experiment, a known EMT inhibitor was successfully evaluated in terms of the changes in cluster area or leader cell appearance, in response to biochemically and mechanically induced EMT. Furthermore, an application of the present method for microbial secondary metabolites identified nanaomycin H as an EMT inhibitor, potentially killing EMTed cells in disseminated conditions. These results demonstrate the potential of the present method for screening new EMT inhibitors.

Original languageEnglish
Pages (from-to)65-69
Number of pages5
JournalAnalytical Sciences
Volume35
Issue number1
DOIs
Publication statusPublished - 2019 Jan 1
Externally publishedYes

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Keywords

  • Caged compound
  • Cancer
  • Collective cell migration
  • Drug screening
  • Epithelial-mesenchymal transition
  • Leader cells
  • Mechanobiology
  • Nanaomycin
  • Patterning
  • Transforming growth factor β

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Nakanishi, J., Sugiyama, K., Matsuo, H., Takahashi, Y. K., Mura, S. O., & Nakashima, T. (2019). An application of photoactivatable substrate for the evaluation of epithelial-mesenchymal transition inhibitors. Analytical Sciences, 35(1), 65-69. https://doi.org/10.2116/analsci.18SDP07