Abstract
Epithelial-mesenchymal transition (EMT), phenotypic changes in cell adhesion and migration, is involved in cancer invasion and metastasis, hence becoming a target for anti-cancer drugs. In this study, we report a method for the evaluation of EMT inhibitors by using a photoactivatable gold substrate, which changes from non-cell-adhesive to celladhesive in response to light. The method is based on the geometrical confinement of cell clusters and the subsequent migration induction by controlled photoirradiation of the substrate. As a proof-of-concept experiment, a known EMT inhibitor was successfully evaluated in terms of the changes in cluster area or leader cell appearance, in response to biochemically and mechanically induced EMT. Furthermore, an application of the present method for microbial secondary metabolites identified nanaomycin H as an EMT inhibitor, potentially killing EMTed cells in disseminated conditions. These results demonstrate the potential of the present method for screening new EMT inhibitors.
| Original language | English |
|---|---|
| Pages (from-to) | 65-69 |
| Number of pages | 5 |
| Journal | Analytical Sciences |
| Volume | 35 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 2019 Jan 1 |
| Externally published | Yes |
Fingerprint
Keywords
- Caged compound
- Cancer
- Collective cell migration
- Drug screening
- Epithelial-mesenchymal transition
- Leader cells
- Mechanobiology
- Nanaomycin
- Patterning
- Transforming growth factor β
ASJC Scopus subject areas
- Analytical Chemistry
Cite this
An application of photoactivatable substrate for the evaluation of epithelial-mesenchymal transition inhibitors. / Nakanishi, Jun; Sugiyama, Kenji; Matsuo, Hirotaka; Takahashi, Yo Ko; Mura, Satoshi O.; Nakashima, Takuji.
In: Analytical Sciences, Vol. 35, No. 1, 01.01.2019, p. 65-69.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - An application of photoactivatable substrate for the evaluation of epithelial-mesenchymal transition inhibitors
AU - Nakanishi, Jun
AU - Sugiyama, Kenji
AU - Matsuo, Hirotaka
AU - Takahashi, Yo Ko
AU - Mura, Satoshi O.
AU - Nakashima, Takuji
PY - 2019/1/1
Y1 - 2019/1/1
N2 - Epithelial-mesenchymal transition (EMT), phenotypic changes in cell adhesion and migration, is involved in cancer invasion and metastasis, hence becoming a target for anti-cancer drugs. In this study, we report a method for the evaluation of EMT inhibitors by using a photoactivatable gold substrate, which changes from non-cell-adhesive to celladhesive in response to light. The method is based on the geometrical confinement of cell clusters and the subsequent migration induction by controlled photoirradiation of the substrate. As a proof-of-concept experiment, a known EMT inhibitor was successfully evaluated in terms of the changes in cluster area or leader cell appearance, in response to biochemically and mechanically induced EMT. Furthermore, an application of the present method for microbial secondary metabolites identified nanaomycin H as an EMT inhibitor, potentially killing EMTed cells in disseminated conditions. These results demonstrate the potential of the present method for screening new EMT inhibitors.
AB - Epithelial-mesenchymal transition (EMT), phenotypic changes in cell adhesion and migration, is involved in cancer invasion and metastasis, hence becoming a target for anti-cancer drugs. In this study, we report a method for the evaluation of EMT inhibitors by using a photoactivatable gold substrate, which changes from non-cell-adhesive to celladhesive in response to light. The method is based on the geometrical confinement of cell clusters and the subsequent migration induction by controlled photoirradiation of the substrate. As a proof-of-concept experiment, a known EMT inhibitor was successfully evaluated in terms of the changes in cluster area or leader cell appearance, in response to biochemically and mechanically induced EMT. Furthermore, an application of the present method for microbial secondary metabolites identified nanaomycin H as an EMT inhibitor, potentially killing EMTed cells in disseminated conditions. These results demonstrate the potential of the present method for screening new EMT inhibitors.
KW - Caged compound
KW - Cancer
KW - Collective cell migration
KW - Drug screening
KW - Epithelial-mesenchymal transition
KW - Leader cells
KW - Mechanobiology
KW - Nanaomycin
KW - Patterning
KW - Transforming growth factor β
UR - http://www.scopus.com/inward/record.url?scp=85059798753&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85059798753&partnerID=8YFLogxK
U2 - 10.2116/analsci.18SDP07
DO - 10.2116/analsci.18SDP07
M3 - Article
VL - 35
SP - 65
EP - 69
JO - Analytical Sciences
JF - Analytical Sciences
SN - 0910-6340
IS - 1
ER -