TY - JOUR
T1 - An approach to imaging of living cell surface topography by scanning tunneling microscopy
AU - Ito, Etsuro
AU - Takahashi, Tetsuo
AU - Hama, Kiyoshi
AU - Yoshioka, Tohru
AU - Mizutani, Wataru
AU - Shimizu, Hajime
AU - Ono, Masatoshi
N1 - Funding Information:
T24 and CHO cells were kind University) and Prof. M. Mishina Messrs. M. Shigeno, E. Tomita, Inoue (Seiko Instruments Inc.) discussion. This research was O224llOl) from the Ministry of ACKNOWLEDGMENTS gifts from Dr. Y. Kubota (Yokohama City (Niigata University), respectively. We thank K. Ishihara, C. Miyata, Y. Shikakura and A. for their technical supports and valuable supported by Grant-in-Aids (6388032 and Education, Science and Culture of Japan.
PY - 1991/6/14
Y1 - 1991/6/14
N2 - Since a scanning tunneling microscope (STM) was developed, an observation of living cell surface has been one of the final aims in the biological application of STM. By developing a new style of STM which was combined with an optical microscope and with a novel system for the centering of both images, we successfully got the STM images of living cell surface of T24 cells (human bladder cancer cell line) and CHO cells (Chinese hamster ovary fibroblast) cultured on highly oriented pyrolytic graphite under the appropriate condition (V > 8.0 V, I < 0.2 nA). Unexpectedly, the living T24 cell showed a slightly uneven surface with a steep foot slope. The CHO cell showed more rough surface with steeper slope of cell foot. Although the STM system had a fine spatial resolution less than 3 nm, the profile of living cell surface covered with electrolyte was clear only when the scanning area was more than 10 μm square.
AB - Since a scanning tunneling microscope (STM) was developed, an observation of living cell surface has been one of the final aims in the biological application of STM. By developing a new style of STM which was combined with an optical microscope and with a novel system for the centering of both images, we successfully got the STM images of living cell surface of T24 cells (human bladder cancer cell line) and CHO cells (Chinese hamster ovary fibroblast) cultured on highly oriented pyrolytic graphite under the appropriate condition (V > 8.0 V, I < 0.2 nA). Unexpectedly, the living T24 cell showed a slightly uneven surface with a steep foot slope. The CHO cell showed more rough surface with steeper slope of cell foot. Although the STM system had a fine spatial resolution less than 3 nm, the profile of living cell surface covered with electrolyte was clear only when the scanning area was more than 10 μm square.
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U2 - 10.1016/0006-291X(91)91836-2
DO - 10.1016/0006-291X(91)91836-2
M3 - Article
C2 - 2049086
AN - SCOPUS:0025864605
SN - 0006-291X
VL - 177
SP - 636
EP - 643
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 2
ER -
