Abstract
The use of synthetic biology to design artificial gene circuits is an important approach for understanding the principles underlying the complicated dynamic behaviours of biomolecular networks, such as genetic switching and biological rhythms. The synthetic approach is also useful in systems biology in that it can be used to create artificial bypasses for processes related to cellular phenomena of interest for their easier analysis. To validate the role of transcription feedback in the cyanobacterial circadian system, we propose an experimental design for a 'semi-synthetic' approach that involves transplantation of the kaiABC genes into Escherichia coli and the construction of chimeric transcriptional outputs. The design principle and preliminary results are discussed.
Original language | English |
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Pages (from-to) | 435-444 |
Number of pages | 10 |
Journal | International Journal of Bioinformatics Research and Applications |
Volume | 4 |
Issue number | 4 |
DOIs | |
Publication status | Published - 2008 Nov |
Keywords
- Circadian
- Escherichia coli
- KaiC
- OmpC
- SasA
ASJC Scopus subject areas
- Biomedical Engineering
- Health Informatics
- Clinical Biochemistry
- Health Information Management