An attempt to reveal a role of a transcription/translation feedback loop in the cyanobacterial KaiC protein-based circadian system by using a semi-synthetic method

Hirokazu Tozaki, Taiichiro Kobe, Kazuyuki Aihara, Hideo Iwasaki

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    Abstract

    The use of synthetic biology to design artificial gene circuits is an important approach for understanding the principles underlying the complicated dynamic behaviours of biomolecular networks, such as genetic switching and biological rhythms. The synthetic approach is also useful in systems biology in that it can be used to create artificial bypasses for processes related to cellular phenomena of interest for their easier analysis. To validate the role of transcription feedback in the cyanobacterial circadian system, we propose an experimental design for a 'semi-synthetic' approach that involves transplantation of the kaiABC genes into Escherichia coli and the construction of chimeric transcriptional outputs. The design principle and preliminary results are discussed.

    Original languageEnglish
    Pages (from-to)435-444
    Number of pages10
    JournalInternational Journal of Bioinformatics Research and Applications
    Volume4
    Issue number4
    DOIs
    Publication statusPublished - 2008 Nov

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    Keywords

    • Circadian
    • Escherichia coli
    • KaiC
    • OmpC
    • SasA

    ASJC Scopus subject areas

    • Health Informatics
    • Health Information Management
    • Biomedical Engineering
    • Clinical Biochemistry

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