Abstract
The use of synthetic biology to design artificial gene circuits is an important approach for understanding the principles underlying the complicated dynamic behaviours of biomolecular networks, such as genetic switching and biological rhythms. The synthetic approach is also useful in systems biology in that it can be used to create artificial bypasses for processes related to cellular phenomena of interest for their easier analysis. To validate the role of transcription feedback in the cyanobacterial circadian system, we propose an experimental design for a 'semi-synthetic' approach that involves transplantation of the kaiABC genes into Escherichia coli and the construction of chimeric transcriptional outputs. The design principle and preliminary results are discussed.
| Original language | English |
|---|---|
| Pages (from-to) | 435-444 |
| Number of pages | 10 |
| Journal | International Journal of Bioinformatics Research and Applications |
| Volume | 4 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - 2008 Nov |
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Keywords
- Circadian
- Escherichia coli
- KaiC
- OmpC
- SasA
ASJC Scopus subject areas
- Health Informatics
- Health Information Management
- Biomedical Engineering
- Clinical Biochemistry
Cite this
An attempt to reveal a role of a transcription/translation feedback loop in the cyanobacterial KaiC protein-based circadian system by using a semi-synthetic method. / Tozaki, Hirokazu; Kobe, Taiichiro; Aihara, Kazuyuki; Iwasaki, Hideo.
In: International Journal of Bioinformatics Research and Applications, Vol. 4, No. 4, 11.2008, p. 435-444.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - An attempt to reveal a role of a transcription/translation feedback loop in the cyanobacterial KaiC protein-based circadian system by using a semi-synthetic method
AU - Tozaki, Hirokazu
AU - Kobe, Taiichiro
AU - Aihara, Kazuyuki
AU - Iwasaki, Hideo
PY - 2008/11
Y1 - 2008/11
N2 - The use of synthetic biology to design artificial gene circuits is an important approach for understanding the principles underlying the complicated dynamic behaviours of biomolecular networks, such as genetic switching and biological rhythms. The synthetic approach is also useful in systems biology in that it can be used to create artificial bypasses for processes related to cellular phenomena of interest for their easier analysis. To validate the role of transcription feedback in the cyanobacterial circadian system, we propose an experimental design for a 'semi-synthetic' approach that involves transplantation of the kaiABC genes into Escherichia coli and the construction of chimeric transcriptional outputs. The design principle and preliminary results are discussed.
AB - The use of synthetic biology to design artificial gene circuits is an important approach for understanding the principles underlying the complicated dynamic behaviours of biomolecular networks, such as genetic switching and biological rhythms. The synthetic approach is also useful in systems biology in that it can be used to create artificial bypasses for processes related to cellular phenomena of interest for their easier analysis. To validate the role of transcription feedback in the cyanobacterial circadian system, we propose an experimental design for a 'semi-synthetic' approach that involves transplantation of the kaiABC genes into Escherichia coli and the construction of chimeric transcriptional outputs. The design principle and preliminary results are discussed.
KW - Circadian
KW - Escherichia coli
KW - KaiC
KW - OmpC
KW - SasA
UR - http://www.scopus.com/inward/record.url?scp=55849096664&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=55849096664&partnerID=8YFLogxK
U2 - 10.1504/IJBRA.2008.021178
DO - 10.1504/IJBRA.2008.021178
M3 - Article
VL - 4
SP - 435
EP - 444
JO - International Journal of Bioinformatics Research and Applications
JF - International Journal of Bioinformatics Research and Applications
SN - 1744-5485
IS - 4
ER -