An attempt to reveal a role of a transcription/translation feedback loop in the cyanobacterial KaiC protein-based circadian system by using a semi-synthetic method

Hirokazu Tozaki, Taiichiro Kobe, Kazuyuki Aihara, Hideo Iwasaki

    Research output: Contribution to journalArticle

    1 Citation (Scopus)

    Abstract

    The use of synthetic biology to design artificial gene circuits is an important approach for understanding the principles underlying the complicated dynamic behaviours of biomolecular networks, such as genetic switching and biological rhythms. The synthetic approach is also useful in systems biology in that it can be used to create artificial bypasses for processes related to cellular phenomena of interest for their easier analysis. To validate the role of transcription feedback in the cyanobacterial circadian system, we propose an experimental design for a 'semi-synthetic' approach that involves transplantation of the kaiABC genes into Escherichia coli and the construction of chimeric transcriptional outputs. The design principle and preliminary results are discussed.

    Original languageEnglish
    Pages (from-to)435-444
    Number of pages10
    JournalInternational Journal of Bioinformatics Research and Applications
    Volume4
    Issue number4
    DOIs
    Publication statusPublished - 2008 Nov

    Fingerprint

    Synthetic Biology
    Synthetic Genes
    Systems Biology
    Gene Regulatory Networks
    Periodicity
    Transcription
    Research Design
    Genes
    Transplantation
    Escherichia coli
    Proteins
    Feedback
    Design of experiments
    Networks (circuits)

    Keywords

    • Circadian
    • Escherichia coli
    • KaiC
    • OmpC
    • SasA

    ASJC Scopus subject areas

    • Health Informatics
    • Health Information Management
    • Biomedical Engineering
    • Clinical Biochemistry

    Cite this

    @article{7bc51265120c4e6182ccb6de7854b42c,
    title = "An attempt to reveal a role of a transcription/translation feedback loop in the cyanobacterial KaiC protein-based circadian system by using a semi-synthetic method",
    abstract = "The use of synthetic biology to design artificial gene circuits is an important approach for understanding the principles underlying the complicated dynamic behaviours of biomolecular networks, such as genetic switching and biological rhythms. The synthetic approach is also useful in systems biology in that it can be used to create artificial bypasses for processes related to cellular phenomena of interest for their easier analysis. To validate the role of transcription feedback in the cyanobacterial circadian system, we propose an experimental design for a 'semi-synthetic' approach that involves transplantation of the kaiABC genes into Escherichia coli and the construction of chimeric transcriptional outputs. The design principle and preliminary results are discussed.",
    keywords = "Circadian, Escherichia coli, KaiC, OmpC, SasA",
    author = "Hirokazu Tozaki and Taiichiro Kobe and Kazuyuki Aihara and Hideo Iwasaki",
    year = "2008",
    month = "11",
    doi = "10.1504/IJBRA.2008.021178",
    language = "English",
    volume = "4",
    pages = "435--444",
    journal = "International Journal of Bioinformatics Research and Applications",
    issn = "1744-5485",
    publisher = "Inderscience Enterprises Ltd",
    number = "4",

    }

    TY - JOUR

    T1 - An attempt to reveal a role of a transcription/translation feedback loop in the cyanobacterial KaiC protein-based circadian system by using a semi-synthetic method

    AU - Tozaki, Hirokazu

    AU - Kobe, Taiichiro

    AU - Aihara, Kazuyuki

    AU - Iwasaki, Hideo

    PY - 2008/11

    Y1 - 2008/11

    N2 - The use of synthetic biology to design artificial gene circuits is an important approach for understanding the principles underlying the complicated dynamic behaviours of biomolecular networks, such as genetic switching and biological rhythms. The synthetic approach is also useful in systems biology in that it can be used to create artificial bypasses for processes related to cellular phenomena of interest for their easier analysis. To validate the role of transcription feedback in the cyanobacterial circadian system, we propose an experimental design for a 'semi-synthetic' approach that involves transplantation of the kaiABC genes into Escherichia coli and the construction of chimeric transcriptional outputs. The design principle and preliminary results are discussed.

    AB - The use of synthetic biology to design artificial gene circuits is an important approach for understanding the principles underlying the complicated dynamic behaviours of biomolecular networks, such as genetic switching and biological rhythms. The synthetic approach is also useful in systems biology in that it can be used to create artificial bypasses for processes related to cellular phenomena of interest for their easier analysis. To validate the role of transcription feedback in the cyanobacterial circadian system, we propose an experimental design for a 'semi-synthetic' approach that involves transplantation of the kaiABC genes into Escherichia coli and the construction of chimeric transcriptional outputs. The design principle and preliminary results are discussed.

    KW - Circadian

    KW - Escherichia coli

    KW - KaiC

    KW - OmpC

    KW - SasA

    UR - http://www.scopus.com/inward/record.url?scp=55849096664&partnerID=8YFLogxK

    UR - http://www.scopus.com/inward/citedby.url?scp=55849096664&partnerID=8YFLogxK

    U2 - 10.1504/IJBRA.2008.021178

    DO - 10.1504/IJBRA.2008.021178

    M3 - Article

    C2 - 19008185

    AN - SCOPUS:55849096664

    VL - 4

    SP - 435

    EP - 444

    JO - International Journal of Bioinformatics Research and Applications

    JF - International Journal of Bioinformatics Research and Applications

    SN - 1744-5485

    IS - 4

    ER -