An Evolutionary Scenario for Gonadotrophin-Inhibitory Hormone in Chordates

T. Osugi, T. Ubuka, Kazuyoshi Tsutsui

    Research output: Contribution to journalArticle

    6 Citations (Scopus)

    Abstract

    In 2000, we discovered a novel hypothalamic neuropeptide that actively inhibits gonadotrophin release in quail and termed it gonadotrophin-inhibitory hormone (GnIH). GnIH peptides have subsequently been identified in most representative species of gnathostomes. They all share a C-terminal LPXRFamide (X = L or Q) motif. GnIH can inhibit gonadotrophin synthesis and release by decreasing the activity of GnRH neuroes, as well as by directly inhibiting pituitary gonadotrophin secretion in birds and mammals. To investigate the evolutionary origin of GnIH and its ancestral function, we identified a GnIH precursor gene encoding GnIHs from the brain of sea lamprey, the most ancient lineage of vertebrates. Lamprey GnIHs possess a C-terminal PQRFamide motif. In vivo administration of one of lamprey GnIHs stimulated the expression of lamprey GnRH in the hypothalamus and gonadotophin β mRNA in the pituitary. Thus, GnIH may have emerged in agnathans as a stimulatory neuropeptide that subsequently diverged to an inhibitory neuropeptide during the course of evolution from basal vertebrates to later-evolved vertebrates, such as birds and mammals. From a structural point of view, pain modulatory neuropeptides, such as neuropeptide FF (NPFF) and neuropeptide AF, share a C-terminal PQRFamide motif. Because agnathans possess both GnIH and NPFF genes, the origin of GnIH and NPFF genes may date back before the emergence of agnathans. More recently, we identified a novel gene encoding RFamide peptides in the amphioxus. Molecular phylogenetic analysis and synteny analysis indicated that this gene is closely related to the genes of GnIH and NPFF of vertebrates. The results suggest that the identified protochordate gene is similar to the common ancestor of GnIH and NPFF genes, indicating that the origin of GnIH and NPFF may date back to the time of the emergence of early chordates. The GnIH and NPFF genes may have diverged by whole-genome duplication during the course of vertebrate evolution.

    Original languageEnglish
    Pages (from-to)556-566
    Number of pages11
    JournalJournal of Neuroendocrinology
    Volume27
    Issue number6
    DOIs
    Publication statusPublished - 2015 Jun 1

    Fingerprint

    Chordata
    Gonadotropins
    Hormones
    Vertebrates
    Genes
    Neuropeptides
    Lampreys
    Gonadotropin-Releasing Hormone
    Birds
    Mammals
    Petromyzon
    Lancelets
    Synteny
    Pituitary Gonadotropins
    Quail
    Peptide Hormones
    phenylalanyl-leucyl-phenylalanyl-glutaminyl-prolyl-glutaminyl-arginyl-phenylalaninamide

    Keywords

    • Amphioxus
    • Chordates
    • Evolution
    • Gonadotrophin-inhibitory hormone
    • Lamprey
    • Reproduction
    • RFamide peptides

    ASJC Scopus subject areas

    • Endocrinology
    • Endocrinology, Diabetes and Metabolism
    • Endocrine and Autonomic Systems
    • Cellular and Molecular Neuroscience

    Cite this

    An Evolutionary Scenario for Gonadotrophin-Inhibitory Hormone in Chordates. / Osugi, T.; Ubuka, T.; Tsutsui, Kazuyoshi.

    In: Journal of Neuroendocrinology, Vol. 27, No. 6, 01.06.2015, p. 556-566.

    Research output: Contribution to journalArticle

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