An exon-trapping system with a newly constructed trapping vector pEXT2; its application to the proximal region of the human chromosome 21 long arm

Naoki Ozawa, Takeshi Kano, Chiharu Taga, Masahira Hattori, Yoshiyuki Sakaki, Harukazu Suzuki

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

We have developed an exon-trapping system with a newly constructed trapping vector containing multiple cloning sites (designated pEXT2). The system revealed high sensitivity for trapping a control exon from several hundred kbp of DNA. We have applied the system to the cosmid clones located on human chromosome 21pll-q21, and identified two fragments highly homologous to neurofibromatosis 1 (NF1) gene and a clearly transcribed fragment hybridized with ~1.6 kb RNA from human brain and human glioblastoma A 172 cell.

Original languageEnglish
Pages (from-to)303-308
Number of pages6
JournalFEBS Letters
Volume325
Issue number3
DOIs
Publication statusPublished - 1993 Jul 5
Externally publishedYes

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Keywords

  • Alzheimer's disease
  • Chromosome 21
  • Gene cloning
  • Neurofibromatosis 1
  • Plasmid vector
  • RNA splicing

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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