An exon-trapping system with a newly constructed trapping vector pEXT2; its application to the proximal region of the human chromosome 21 long arm

Naoki Ozawa, Takeshi Kano, Chiharu Taga, Masahira Hattori, Yoshiyuki Sakaki, Harukazu Suzuki

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

We have developed an exon-trapping system with a newly constructed trapping vector containing multiple cloning sites (designated pEXT2). The system revealed high sensitivity for trapping a control exon from several hundred kbp of DNA. We have applied the system to the cosmid clones located on human chromosome 21pll-q21, and identified two fragments highly homologous to neurofibromatosis 1 (NF1) gene and a clearly transcribed fragment hybridized with ~1.6 kb RNA from human brain and human glioblastoma A 172 cell.

Original languageEnglish
Pages (from-to)303-308
Number of pages6
JournalFEBS Letters
Volume325
Issue number3
DOIs
Publication statusPublished - 1993 Jul 5
Externally publishedYes

Fingerprint

Chromosomes, Human, Pair 21
Human Chromosomes
Chromosomes
Exons
Neurofibromatosis 1 Genes
Cosmids
Cloning
Glioblastoma
Organism Cloning
Brain
Clone Cells
Genes
RNA
DNA

Keywords

  • Alzheimer's disease
  • Chromosome 21
  • Gene cloning
  • Neurofibromatosis 1
  • Plasmid vector
  • RNA splicing

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

An exon-trapping system with a newly constructed trapping vector pEXT2; its application to the proximal region of the human chromosome 21 long arm. / Ozawa, Naoki; Kano, Takeshi; Taga, Chiharu; Hattori, Masahira; Sakaki, Yoshiyuki; Suzuki, Harukazu.

In: FEBS Letters, Vol. 325, No. 3, 05.07.1993, p. 303-308.

Research output: Contribution to journalArticle

Ozawa, Naoki ; Kano, Takeshi ; Taga, Chiharu ; Hattori, Masahira ; Sakaki, Yoshiyuki ; Suzuki, Harukazu. / An exon-trapping system with a newly constructed trapping vector pEXT2; its application to the proximal region of the human chromosome 21 long arm. In: FEBS Letters. 1993 ; Vol. 325, No. 3. pp. 303-308.
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