An extended gate FET-based biosensor integrated with a Si microfluidic channel for detection of protein complexes

Dong Sun Kim, Jee Eun Park, Jang Kyoo Shin, Pan Kyeom Kim, Geunbae Lim, Shuichi Shoji

    Research output: Contribution to journalArticle

    70 Citations (Scopus)

    Abstract

    In this work, we present an extended gate field effect transistor (EGFET)-based biosensor integrated with a silicon micro-fluidic channel for the electronic detection of streptavidin-biotin protein complexes. The connection between the EGFET and microfluidic system could be achieved with the proposed device, as it offers isolation between the device and solution, compatibility with the integrated circuit (IC) technology and, is applicable to the micro total analysis system (μ-TAS). The device was fabricated on the basis of semiconductor IC fabrication and micro-electro mechanical system (MEMS) technology. Au was used as the extended gate metal to form a self-assembled monolayer (SAM) with thiol. The bindings of the SAM, streptavidin and biotin were detected by measuring the electrical characteristics of the FET device. We also verified the interactions among the SAM, streptavidin, and biotin by using surface plasmon resonance (SPR) measurements. Furthermore, atomic force microscopy (AFM) images of the bio-layers formed on the Au electrode were taken in a solution in order to determine the presence of protein biomolecules with the proposed configuration.

    Original languageEnglish
    Pages (from-to)488-494
    Number of pages7
    JournalSensors and Actuators, B: Chemical
    Volume117
    Issue number2
    DOIs
    Publication statusPublished - 2006 Oct 12

    Fingerprint

    Streptavidin
    Self assembled monolayers
    Biotin
    Field effect transistors
    bioinstrumentation
    Microfluidics
    Biosensors
    biotin
    Gates (transistor)
    field effect transistors
    proteins
    Proteins
    Integrated circuits
    integrated circuits
    Fluidics
    Surface plasmon resonance
    Biomolecules
    Silicon
    Sulfhydryl Compounds
    Atomic force microscopy

    Keywords

    • Bio-AFM
    • EGFET
    • FET-type biosensor
    • Microfluidic channel
    • Protein sensor
    • SPR

    ASJC Scopus subject areas

    • Analytical Chemistry
    • Electrochemistry
    • Electrical and Electronic Engineering

    Cite this

    An extended gate FET-based biosensor integrated with a Si microfluidic channel for detection of protein complexes. / Kim, Dong Sun; Park, Jee Eun; Shin, Jang Kyoo; Kim, Pan Kyeom; Lim, Geunbae; Shoji, Shuichi.

    In: Sensors and Actuators, B: Chemical, Vol. 117, No. 2, 12.10.2006, p. 488-494.

    Research output: Contribution to journalArticle

    Kim, Dong Sun ; Park, Jee Eun ; Shin, Jang Kyoo ; Kim, Pan Kyeom ; Lim, Geunbae ; Shoji, Shuichi. / An extended gate FET-based biosensor integrated with a Si microfluidic channel for detection of protein complexes. In: Sensors and Actuators, B: Chemical. 2006 ; Vol. 117, No. 2. pp. 488-494.
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