An in vitro circadian rhythm of protein synthesis in the rat suprachiasmatic nucleus under tissue culture conditions

Shigenobu Shibata, Toshiyuki Hamada, Keiko Tominaga, Shigenori Watanabe

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Because inhibitors of protein synthesis produce changes in the circadian rhythm of wheel-running activity in rodents, we examined the circadian changes of in vitro protein synthesis by the rat suprachiasmatic nucleus (SCN). We demonstrated a robust circadian rhythm of [14C]leucine incorporation as well as [3H]2-deoxyglucose (2DG) uptake by the SCN. The peak time of 2DG uptake was around circadian time 6-9 h (CT6-CT9). In contrast to 2DG uptake, the maximum rate of leucine incorporation occurred around CT22-CT0. Thus, the leucine incorporation rate preceded 2DG uptake by 6-9 h. Leucine incorporation was inhibited by protein synthesis inhibitors, but not by tetrodotoxin. Since a robust circadian rhythm of leucine incorporation by the SCN was detected in vitro by using our method, this procedure may be useful to study the circadian clock function of the SCN.

Original languageEnglish
Pages (from-to)251-256
Number of pages6
JournalBrain Research
Volume584
Issue number1-2
DOIs
Publication statusPublished - 1992 Jul 3
Externally publishedYes

Fingerprint

Suprachiasmatic Nucleus
Circadian Rhythm
Leucine
Deoxyglucose
Protein Synthesis Inhibitors
Proteins
Circadian Clocks
Tetrodotoxin
Running
Rodentia
In Vitro Techniques

Keywords

  • Circadian rhythm
  • Protein synthesis
  • Suprachiasmatic nucleus
  • tissue culture

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology
  • Clinical Neurology
  • Neuroscience(all)

Cite this

An in vitro circadian rhythm of protein synthesis in the rat suprachiasmatic nucleus under tissue culture conditions. / Shibata, Shigenobu; Hamada, Toshiyuki; Tominaga, Keiko; Watanabe, Shigenori.

In: Brain Research, Vol. 584, No. 1-2, 03.07.1992, p. 251-256.

Research output: Contribution to journalArticle

Shibata, Shigenobu ; Hamada, Toshiyuki ; Tominaga, Keiko ; Watanabe, Shigenori. / An in vitro circadian rhythm of protein synthesis in the rat suprachiasmatic nucleus under tissue culture conditions. In: Brain Research. 1992 ; Vol. 584, No. 1-2. pp. 251-256.
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