An increase in circulating mast cell colony-forming cells in asthma

H. H. Mwamtemi, K. Koike, T. Kinoshita, S. Ito, S. Ishida, Y. Nakazawa, Y. Kurokawa, K. Shinozaki, K. Sakashita, K. Takeuchi, M. Shiohara, T. Kamijo, Y. Yasui, A. Ishiguro, Y. Kawano, K. Kitano, H. Miyazaki, Takashi Kato, S. Sakuma, A. Komiyama

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

We compared a potential to generate mast cells among various sources of CD34+ peripheral blood (PB) cells in the presence of stem cell factor (SCF) with or without thrombopoietin (TPO), using a serum-deprived liquid culture system. From the time course of relative numbers of tryptase-positive and chymase-positive cells in the cultured cells grown by CD34+ PB cells of nonasthmatic healthy individuals treated with G-CSF, TPO appears to potentiate the SCF-dependent growth of mast cells without influencing the differentiation into mast cell lineage. CD34+ PB cells from asthmatic patients in a stable condition generated significantly more mast cells under stimulation with SCF alone or SCF+TPO at 6 wk of culture than did steady-state CD34+ PB cells of normal controls. Single-cell culture studies showed a substantial difference in the number of SCF-responsive or SCF+TPO-responsive mast cell progenitors in CD34+ PB cells between the two groups. In the presence of TPO, CD34+ PB cells from asthmatic children could respond to a suboptimal concentration of SCF to a greater extent, compared with the values obtained by those of normal controls. Six-week cultured mast cells of asthmatic subjects had maturation properties (intracellular histamine content and tryptase/chymase enzymatic activities) similar to those derived from mobilized CD34+ PB cells of nonasthmatic subjects. An increase in a potential of circulating hemopoietic progenitors to differentiate into mast cell lineage may contribute to the recruitment of mast cells toward sites of asthmatic mucosal inflammation.

Original languageEnglish
Pages (from-to)4672-4677
Number of pages6
JournalJournal of Immunology
Volume166
Issue number7
Publication statusPublished - 2001 Apr 1
Externally publishedYes

Fingerprint

Stem Cell Factor
Mast Cells
Thrombopoietin
Asthma
Blood Cells
Chymases
Tryptases
Cell Lineage
Cultured Cells
Granulocyte Colony-Stimulating Factor
Histamine
Cell Culture Techniques
Inflammation
Growth
Serum

ASJC Scopus subject areas

  • Immunology

Cite this

Mwamtemi, H. H., Koike, K., Kinoshita, T., Ito, S., Ishida, S., Nakazawa, Y., ... Komiyama, A. (2001). An increase in circulating mast cell colony-forming cells in asthma. Journal of Immunology, 166(7), 4672-4677.

An increase in circulating mast cell colony-forming cells in asthma. / Mwamtemi, H. H.; Koike, K.; Kinoshita, T.; Ito, S.; Ishida, S.; Nakazawa, Y.; Kurokawa, Y.; Shinozaki, K.; Sakashita, K.; Takeuchi, K.; Shiohara, M.; Kamijo, T.; Yasui, Y.; Ishiguro, A.; Kawano, Y.; Kitano, K.; Miyazaki, H.; Kato, Takashi; Sakuma, S.; Komiyama, A.

In: Journal of Immunology, Vol. 166, No. 7, 01.04.2001, p. 4672-4677.

Research output: Contribution to journalArticle

Mwamtemi, HH, Koike, K, Kinoshita, T, Ito, S, Ishida, S, Nakazawa, Y, Kurokawa, Y, Shinozaki, K, Sakashita, K, Takeuchi, K, Shiohara, M, Kamijo, T, Yasui, Y, Ishiguro, A, Kawano, Y, Kitano, K, Miyazaki, H, Kato, T, Sakuma, S & Komiyama, A 2001, 'An increase in circulating mast cell colony-forming cells in asthma', Journal of Immunology, vol. 166, no. 7, pp. 4672-4677.
Mwamtemi HH, Koike K, Kinoshita T, Ito S, Ishida S, Nakazawa Y et al. An increase in circulating mast cell colony-forming cells in asthma. Journal of Immunology. 2001 Apr 1;166(7):4672-4677.
Mwamtemi, H. H. ; Koike, K. ; Kinoshita, T. ; Ito, S. ; Ishida, S. ; Nakazawa, Y. ; Kurokawa, Y. ; Shinozaki, K. ; Sakashita, K. ; Takeuchi, K. ; Shiohara, M. ; Kamijo, T. ; Yasui, Y. ; Ishiguro, A. ; Kawano, Y. ; Kitano, K. ; Miyazaki, H. ; Kato, Takashi ; Sakuma, S. ; Komiyama, A. / An increase in circulating mast cell colony-forming cells in asthma. In: Journal of Immunology. 2001 ; Vol. 166, No. 7. pp. 4672-4677.
@article{43a5320983694ec382cde15208bad066,
title = "An increase in circulating mast cell colony-forming cells in asthma",
abstract = "We compared a potential to generate mast cells among various sources of CD34+ peripheral blood (PB) cells in the presence of stem cell factor (SCF) with or without thrombopoietin (TPO), using a serum-deprived liquid culture system. From the time course of relative numbers of tryptase-positive and chymase-positive cells in the cultured cells grown by CD34+ PB cells of nonasthmatic healthy individuals treated with G-CSF, TPO appears to potentiate the SCF-dependent growth of mast cells without influencing the differentiation into mast cell lineage. CD34+ PB cells from asthmatic patients in a stable condition generated significantly more mast cells under stimulation with SCF alone or SCF+TPO at 6 wk of culture than did steady-state CD34+ PB cells of normal controls. Single-cell culture studies showed a substantial difference in the number of SCF-responsive or SCF+TPO-responsive mast cell progenitors in CD34+ PB cells between the two groups. In the presence of TPO, CD34+ PB cells from asthmatic children could respond to a suboptimal concentration of SCF to a greater extent, compared with the values obtained by those of normal controls. Six-week cultured mast cells of asthmatic subjects had maturation properties (intracellular histamine content and tryptase/chymase enzymatic activities) similar to those derived from mobilized CD34+ PB cells of nonasthmatic subjects. An increase in a potential of circulating hemopoietic progenitors to differentiate into mast cell lineage may contribute to the recruitment of mast cells toward sites of asthmatic mucosal inflammation.",
author = "Mwamtemi, {H. H.} and K. Koike and T. Kinoshita and S. Ito and S. Ishida and Y. Nakazawa and Y. Kurokawa and K. Shinozaki and K. Sakashita and K. Takeuchi and M. Shiohara and T. Kamijo and Y. Yasui and A. Ishiguro and Y. Kawano and K. Kitano and H. Miyazaki and Takashi Kato and S. Sakuma and A. Komiyama",
year = "2001",
month = "4",
day = "1",
language = "English",
volume = "166",
pages = "4672--4677",
journal = "Journal of Immunology",
issn = "0022-1767",
publisher = "American Association of Immunologists",
number = "7",

}

TY - JOUR

T1 - An increase in circulating mast cell colony-forming cells in asthma

AU - Mwamtemi, H. H.

AU - Koike, K.

AU - Kinoshita, T.

AU - Ito, S.

AU - Ishida, S.

AU - Nakazawa, Y.

AU - Kurokawa, Y.

AU - Shinozaki, K.

AU - Sakashita, K.

AU - Takeuchi, K.

AU - Shiohara, M.

AU - Kamijo, T.

AU - Yasui, Y.

AU - Ishiguro, A.

AU - Kawano, Y.

AU - Kitano, K.

AU - Miyazaki, H.

AU - Kato, Takashi

AU - Sakuma, S.

AU - Komiyama, A.

PY - 2001/4/1

Y1 - 2001/4/1

N2 - We compared a potential to generate mast cells among various sources of CD34+ peripheral blood (PB) cells in the presence of stem cell factor (SCF) with or without thrombopoietin (TPO), using a serum-deprived liquid culture system. From the time course of relative numbers of tryptase-positive and chymase-positive cells in the cultured cells grown by CD34+ PB cells of nonasthmatic healthy individuals treated with G-CSF, TPO appears to potentiate the SCF-dependent growth of mast cells without influencing the differentiation into mast cell lineage. CD34+ PB cells from asthmatic patients in a stable condition generated significantly more mast cells under stimulation with SCF alone or SCF+TPO at 6 wk of culture than did steady-state CD34+ PB cells of normal controls. Single-cell culture studies showed a substantial difference in the number of SCF-responsive or SCF+TPO-responsive mast cell progenitors in CD34+ PB cells between the two groups. In the presence of TPO, CD34+ PB cells from asthmatic children could respond to a suboptimal concentration of SCF to a greater extent, compared with the values obtained by those of normal controls. Six-week cultured mast cells of asthmatic subjects had maturation properties (intracellular histamine content and tryptase/chymase enzymatic activities) similar to those derived from mobilized CD34+ PB cells of nonasthmatic subjects. An increase in a potential of circulating hemopoietic progenitors to differentiate into mast cell lineage may contribute to the recruitment of mast cells toward sites of asthmatic mucosal inflammation.

AB - We compared a potential to generate mast cells among various sources of CD34+ peripheral blood (PB) cells in the presence of stem cell factor (SCF) with or without thrombopoietin (TPO), using a serum-deprived liquid culture system. From the time course of relative numbers of tryptase-positive and chymase-positive cells in the cultured cells grown by CD34+ PB cells of nonasthmatic healthy individuals treated with G-CSF, TPO appears to potentiate the SCF-dependent growth of mast cells without influencing the differentiation into mast cell lineage. CD34+ PB cells from asthmatic patients in a stable condition generated significantly more mast cells under stimulation with SCF alone or SCF+TPO at 6 wk of culture than did steady-state CD34+ PB cells of normal controls. Single-cell culture studies showed a substantial difference in the number of SCF-responsive or SCF+TPO-responsive mast cell progenitors in CD34+ PB cells between the two groups. In the presence of TPO, CD34+ PB cells from asthmatic children could respond to a suboptimal concentration of SCF to a greater extent, compared with the values obtained by those of normal controls. Six-week cultured mast cells of asthmatic subjects had maturation properties (intracellular histamine content and tryptase/chymase enzymatic activities) similar to those derived from mobilized CD34+ PB cells of nonasthmatic subjects. An increase in a potential of circulating hemopoietic progenitors to differentiate into mast cell lineage may contribute to the recruitment of mast cells toward sites of asthmatic mucosal inflammation.

UR - http://www.scopus.com/inward/record.url?scp=0035313129&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035313129&partnerID=8YFLogxK

M3 - Article

C2 - 11254727

AN - SCOPUS:0035313129

VL - 166

SP - 4672

EP - 4677

JO - Journal of Immunology

JF - Journal of Immunology

SN - 0022-1767

IS - 7

ER -