An interspecies comparison of placental gangliosides

Saki Itonori, Mitsunari Fukuro, Motoi Yoshioka, Tadashi Tai, Nobuo Sasaki, Teruo Tobayama, Kunio Shiota, Tomoya Ogawa

Research output: Contribution to journalArticle

Abstract

The major ganglioside compositions of placental tissues from several species (rat, sea lion, porpoise, cow and horse) were compared. The samples were extracted with chloroform/ methanol/water and the glycosphingolipid fractions were isolated through sequential treatment by peracetylation with pyridine/acetic anhydride, Florisil column chromatography, and deacetylation with sodium hydroxide. Gangliosides were isolated using DEAE Sephadex A-25 column chromatography. Their structures were identified by silica-gel thin-layer chromatography (TLC) and TLC/ immunostaining using eight monoclonal antibodies that specifically recognize GM3, GM2, GD3, GD1a and IV3NeuAc-nLc 4Cer (SPG). The major ganglioside in each placenta was GM3, comprising 50-80% of the total gangliosides. GM3 containing N-acetylneuraminic acid (NeuAc) was the major placental ganglioside in the sea lion and horse, whereas GM3 containing N-glycolylneuraminic acid (NeuGc) was the major ganglioside in the porpoise. In rat and cow placentas, the expression levels of both types of GM3 were equal. GD3 was detected in all five species. SPG was detected in them all except the rat. TLC/immunostaining detected GM2 (NeuAc) in the sea lion and cow, and GD1a (NeuAc, NeuAc) in the sea lion. We previously reported that the major gangliosides present in the rat placenta were GM3 and GD3. Therefore, GM3 and GD3 was commonly expressed in the placenta of six animal species examined.

Original languageEnglish
Pages (from-to)139-146
Number of pages8
JournalJournal of Reproduction and Development
Volume42
Issue number2
Publication statusPublished - 1996
Externally publishedYes

Fingerprint

gangliosides
Otariidae
placenta
thin layer chromatography
acids
Phocoenidae
rats
chromatography
Sirenia
glycosphingolipids
horses
cows
pyridines
sodium hydroxide
silica gel
chloroform
monoclonal antibodies
methanol

Keywords

  • Cow
  • Gangliosides
  • Horse
  • Placenta
  • Porpoise
  • Rat
  • Sea lion

ASJC Scopus subject areas

  • Animal Science and Zoology

Cite this

Itonori, S., Fukuro, M., Yoshioka, M., Tai, T., Sasaki, N., Tobayama, T., ... Ogawa, T. (1996). An interspecies comparison of placental gangliosides. Journal of Reproduction and Development, 42(2), 139-146.

An interspecies comparison of placental gangliosides. / Itonori, Saki; Fukuro, Mitsunari; Yoshioka, Motoi; Tai, Tadashi; Sasaki, Nobuo; Tobayama, Teruo; Shiota, Kunio; Ogawa, Tomoya.

In: Journal of Reproduction and Development, Vol. 42, No. 2, 1996, p. 139-146.

Research output: Contribution to journalArticle

Itonori, S, Fukuro, M, Yoshioka, M, Tai, T, Sasaki, N, Tobayama, T, Shiota, K & Ogawa, T 1996, 'An interspecies comparison of placental gangliosides', Journal of Reproduction and Development, vol. 42, no. 2, pp. 139-146.
Itonori S, Fukuro M, Yoshioka M, Tai T, Sasaki N, Tobayama T et al. An interspecies comparison of placental gangliosides. Journal of Reproduction and Development. 1996;42(2):139-146.
Itonori, Saki ; Fukuro, Mitsunari ; Yoshioka, Motoi ; Tai, Tadashi ; Sasaki, Nobuo ; Tobayama, Teruo ; Shiota, Kunio ; Ogawa, Tomoya. / An interspecies comparison of placental gangliosides. In: Journal of Reproduction and Development. 1996 ; Vol. 42, No. 2. pp. 139-146.
@article{ec8427dbc8e94b3d96d4aec68c43643c,
title = "An interspecies comparison of placental gangliosides",
abstract = "The major ganglioside compositions of placental tissues from several species (rat, sea lion, porpoise, cow and horse) were compared. The samples were extracted with chloroform/ methanol/water and the glycosphingolipid fractions were isolated through sequential treatment by peracetylation with pyridine/acetic anhydride, Florisil column chromatography, and deacetylation with sodium hydroxide. Gangliosides were isolated using DEAE Sephadex A-25 column chromatography. Their structures were identified by silica-gel thin-layer chromatography (TLC) and TLC/ immunostaining using eight monoclonal antibodies that specifically recognize GM3, GM2, GD3, GD1a and IV3NeuAc-nLc 4Cer (SPG). The major ganglioside in each placenta was GM3, comprising 50-80{\%} of the total gangliosides. GM3 containing N-acetylneuraminic acid (NeuAc) was the major placental ganglioside in the sea lion and horse, whereas GM3 containing N-glycolylneuraminic acid (NeuGc) was the major ganglioside in the porpoise. In rat and cow placentas, the expression levels of both types of GM3 were equal. GD3 was detected in all five species. SPG was detected in them all except the rat. TLC/immunostaining detected GM2 (NeuAc) in the sea lion and cow, and GD1a (NeuAc, NeuAc) in the sea lion. We previously reported that the major gangliosides present in the rat placenta were GM3 and GD3. Therefore, GM3 and GD3 was commonly expressed in the placenta of six animal species examined.",
keywords = "Cow, Gangliosides, Horse, Placenta, Porpoise, Rat, Sea lion",
author = "Saki Itonori and Mitsunari Fukuro and Motoi Yoshioka and Tadashi Tai and Nobuo Sasaki and Teruo Tobayama and Kunio Shiota and Tomoya Ogawa",
year = "1996",
language = "English",
volume = "42",
pages = "139--146",
journal = "Journal of Reproduction and Development",
issn = "0916-8818",
publisher = "Japanese Society of Animal Reproduction (JSAR)",
number = "2",

}

TY - JOUR

T1 - An interspecies comparison of placental gangliosides

AU - Itonori, Saki

AU - Fukuro, Mitsunari

AU - Yoshioka, Motoi

AU - Tai, Tadashi

AU - Sasaki, Nobuo

AU - Tobayama, Teruo

AU - Shiota, Kunio

AU - Ogawa, Tomoya

PY - 1996

Y1 - 1996

N2 - The major ganglioside compositions of placental tissues from several species (rat, sea lion, porpoise, cow and horse) were compared. The samples were extracted with chloroform/ methanol/water and the glycosphingolipid fractions were isolated through sequential treatment by peracetylation with pyridine/acetic anhydride, Florisil column chromatography, and deacetylation with sodium hydroxide. Gangliosides were isolated using DEAE Sephadex A-25 column chromatography. Their structures were identified by silica-gel thin-layer chromatography (TLC) and TLC/ immunostaining using eight monoclonal antibodies that specifically recognize GM3, GM2, GD3, GD1a and IV3NeuAc-nLc 4Cer (SPG). The major ganglioside in each placenta was GM3, comprising 50-80% of the total gangliosides. GM3 containing N-acetylneuraminic acid (NeuAc) was the major placental ganglioside in the sea lion and horse, whereas GM3 containing N-glycolylneuraminic acid (NeuGc) was the major ganglioside in the porpoise. In rat and cow placentas, the expression levels of both types of GM3 were equal. GD3 was detected in all five species. SPG was detected in them all except the rat. TLC/immunostaining detected GM2 (NeuAc) in the sea lion and cow, and GD1a (NeuAc, NeuAc) in the sea lion. We previously reported that the major gangliosides present in the rat placenta were GM3 and GD3. Therefore, GM3 and GD3 was commonly expressed in the placenta of six animal species examined.

AB - The major ganglioside compositions of placental tissues from several species (rat, sea lion, porpoise, cow and horse) were compared. The samples were extracted with chloroform/ methanol/water and the glycosphingolipid fractions were isolated through sequential treatment by peracetylation with pyridine/acetic anhydride, Florisil column chromatography, and deacetylation with sodium hydroxide. Gangliosides were isolated using DEAE Sephadex A-25 column chromatography. Their structures were identified by silica-gel thin-layer chromatography (TLC) and TLC/ immunostaining using eight monoclonal antibodies that specifically recognize GM3, GM2, GD3, GD1a and IV3NeuAc-nLc 4Cer (SPG). The major ganglioside in each placenta was GM3, comprising 50-80% of the total gangliosides. GM3 containing N-acetylneuraminic acid (NeuAc) was the major placental ganglioside in the sea lion and horse, whereas GM3 containing N-glycolylneuraminic acid (NeuGc) was the major ganglioside in the porpoise. In rat and cow placentas, the expression levels of both types of GM3 were equal. GD3 was detected in all five species. SPG was detected in them all except the rat. TLC/immunostaining detected GM2 (NeuAc) in the sea lion and cow, and GD1a (NeuAc, NeuAc) in the sea lion. We previously reported that the major gangliosides present in the rat placenta were GM3 and GD3. Therefore, GM3 and GD3 was commonly expressed in the placenta of six animal species examined.

KW - Cow

KW - Gangliosides

KW - Horse

KW - Placenta

KW - Porpoise

KW - Rat

KW - Sea lion

UR - http://www.scopus.com/inward/record.url?scp=53349160348&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=53349160348&partnerID=8YFLogxK

M3 - Article

VL - 42

SP - 139

EP - 146

JO - Journal of Reproduction and Development

JF - Journal of Reproduction and Development

SN - 0916-8818

IS - 2

ER -