An iron-regulated gene, magA, encoding an iron transport protein of Magnetospirillum sp. strain AMB-1

C. Nakamura, J. G. Burgess, K. Sode, Tadashi Matsunaga

Research output: Contribution to journalArticle

134 Citations (Scopus)

Abstract

Magnetospirillum sp. AMB-1 is a freshwater magnetic bacterium which synthesizes intracellular particles of magnetite (Fe3O4). A genomic DNA fragment required for synthesis of magnetic particles was previously isolated from a nonmagnetic transposon Tn5 mutant. We have determined the complete nucleotide sequence of this fragment. The 2975-base pair region contains two putative open reading frames. One open reading frame, designated magA, encodes a polypeptide which is homologous to the cation efflux proteins, the Escherichia coli potassium ion-translocating protein, KefC, and the putative Na+/H+-antiporter, NapA, from Enterococcus hirae. Northern hybridization demonstrated that the magA mRNA transcript is 1.3 kilobases in size, corresponding to the size of the magA gene. A functional promoter was located upstream from the magA gene, and the transcription in AMB-1 was regulated by environmental iron concentration. Vesicles isolated from E. coli in which the MagA protein was expressed exhibited iron accumulation ability. We consider that the MagA protein is an iron transport involved in the synthesis of magnetic particles in AMB-1.

Original languageEnglish
Pages (from-to)28392-28396
Number of pages5
JournalJournal of Biological Chemistry
Volume270
Issue number47
DOIs
Publication statusPublished - 1995
Externally publishedYes

Fingerprint

Magnetospirillum
Gene encoding
Carrier Proteins
Iron
Open Reading Frames
Genes
Ferrosoferric Oxide
Sodium-Hydrogen Antiporter
Proteins
Escherichia coli Proteins
Transcription
Fresh Water
Base Pairing
Escherichia coli
Cations
Bacteria
Potassium
Nucleotides
Ions
Messenger RNA

ASJC Scopus subject areas

  • Biochemistry

Cite this

An iron-regulated gene, magA, encoding an iron transport protein of Magnetospirillum sp. strain AMB-1. / Nakamura, C.; Burgess, J. G.; Sode, K.; Matsunaga, Tadashi.

In: Journal of Biological Chemistry, Vol. 270, No. 47, 1995, p. 28392-28396.

Research output: Contribution to journalArticle

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AU - Matsunaga, Tadashi

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