An organic transistor matrix for multipoint intracellular action potential recording

Yasutoshi Jimbo, Daisuke Sasaki, Takashi Ohya, Sunghoon Lee, Wonryung Lee, Faezeh Arab Hassani, Tomoyuki Yokota, Katsuhisa Matsuura, Shinjiro Umezu, Tatsuya Shimizu, Takao Someya*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Electrode arrays are widely used for multipoint recording of electrophysiological activities, and organic electronics have been utilized to achieve both high performance and biocompatibility. However, extracellular electrode arrays record the field potential instead of the membrane potential itself, resulting in the loss of information and signal amplitude. Although much effort has been dedicated to developing intracellular access methods, their threedimensional structures and advanced protocols prohibited implementation with organic electronics. Here, we show an organic electrochemical transistor (OECT) matrix for the intracellular action potential recording. The driving voltage of sensor matrix simultaneously causes electroporation so that intracellular action potentials are recorded with simple equipment. The amplitude of the recorded peaks was larger than that of an extracellular field potential recording, and it was further enhanced by tuning the driving voltage and geometry of OECTs. The capability of miniaturization and multiplexed recording was demonstrated through a 4 × 4 action potential mapping using a matrix of 5- × 5-μm2 OECTs. Those features are realized using a mild fabrication process and a simple circuit without limiting the potential applications of functional organic electronics.

Original languageEnglish
Article numbere2022300118
JournalProceedings of the National Academy of Sciences of the United States of America
Volume118
Issue number39
DOIs
Publication statusPublished - 2021 Sep 28

Keywords

  • Action potential mapping
  • Biomonitoring interface
  • Intracellular recording
  • Organic electrochemical transistor
  • Organic electronics

ASJC Scopus subject areas

  • General

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