An RNA-interacting protein, SYNCRIP (heterogeneous nuclear ribonuclear protein Q1/NSAP1) is a component of mRNA granule transported with inositol 1,4,5-trisphosphate receptor type 1 mRNA in neuronal dendrites

Hiroko Bannai, Kazumi Fukatsu, Akihiro Mizutani, Tohru Natsume, Shuin Ichiro Iemura, Tohru Ikegami, Takafumi Inoue, Katsuhiko Mikoshiba

Research output: Contribution to journalArticle

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Abstract

mRNA transport and local translation in the neuronal dendrite is implicated in the induction of synaptic plasticity. Recently, we cloned an RNA-interacting protein, SYNCRIP (heterogeneous nuclear ribonuclear protein Q1/NSAP1), that is suggested to be important for the stabilization of mRNA. We report here that SYNCRIP is a component of mRNA granules in rat hippocampal neurons. SYNCRIP was mainly found at cell bodies, but punctate expression patterns in the proximal dendrite were also seen. Time-lapse analysis in living neurons revealed that the granules labeled with fluorescent protein-tagged SYNCRIP were transported bi-directionally within the dendrite at ∼0.05 μm/s. Treatment of neurons with nocodazole significantly inhibited the movement of green fluorescent protein-SYNCRIP-positive granules, indicating that the transport of SYNCRIP-containing granules is dependent on microtubules. The distribution of SYNCRIP-containing granules overlapped with that of dendritic RNAs and elongation factor 1α. SYNCRIP was also found to be co-transported with green fluorescent protein-tagged human staufen1 and the 3′-untranslated region of inositol 1,4,5-trisphosphate receptor type 1 mRNA. These results suggest that SYNCRIP is transported within the dendrite as a component of mRNA granules and raise the possibility that mRNA turnover in mRNA granules and the regulation of local protein synthesis in neuronal dendrites may involve SYNCRIP.

Original languageEnglish
Pages (from-to)53427-53434
Number of pages8
JournalJournal of Biological Chemistry
Volume279
Issue number51
DOIs
Publication statusPublished - 2004 Dec 17
Externally publishedYes

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Inositol 1,4,5-Trisphosphate Receptors
Nuclear Proteins
Dendrites
RNA
Messenger RNA
Proteins
Neurons
Green Fluorescent Proteins
Peptide Elongation Factor 1
Nocodazole
Neuronal Plasticity
3' Untranslated Regions
Microtubules
Plasticity
Rats
Stabilization
Cells

ASJC Scopus subject areas

  • Biochemistry

Cite this

An RNA-interacting protein, SYNCRIP (heterogeneous nuclear ribonuclear protein Q1/NSAP1) is a component of mRNA granule transported with inositol 1,4,5-trisphosphate receptor type 1 mRNA in neuronal dendrites. / Bannai, Hiroko; Fukatsu, Kazumi; Mizutani, Akihiro; Natsume, Tohru; Iemura, Shuin Ichiro; Ikegami, Tohru; Inoue, Takafumi; Mikoshiba, Katsuhiko.

In: Journal of Biological Chemistry, Vol. 279, No. 51, 17.12.2004, p. 53427-53434.

Research output: Contribution to journalArticle

Bannai, Hiroko ; Fukatsu, Kazumi ; Mizutani, Akihiro ; Natsume, Tohru ; Iemura, Shuin Ichiro ; Ikegami, Tohru ; Inoue, Takafumi ; Mikoshiba, Katsuhiko. / An RNA-interacting protein, SYNCRIP (heterogeneous nuclear ribonuclear protein Q1/NSAP1) is a component of mRNA granule transported with inositol 1,4,5-trisphosphate receptor type 1 mRNA in neuronal dendrites. In: Journal of Biological Chemistry. 2004 ; Vol. 279, No. 51. pp. 53427-53434.
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abstract = "mRNA transport and local translation in the neuronal dendrite is implicated in the induction of synaptic plasticity. Recently, we cloned an RNA-interacting protein, SYNCRIP (heterogeneous nuclear ribonuclear protein Q1/NSAP1), that is suggested to be important for the stabilization of mRNA. We report here that SYNCRIP is a component of mRNA granules in rat hippocampal neurons. SYNCRIP was mainly found at cell bodies, but punctate expression patterns in the proximal dendrite were also seen. Time-lapse analysis in living neurons revealed that the granules labeled with fluorescent protein-tagged SYNCRIP were transported bi-directionally within the dendrite at ∼0.05 μm/s. Treatment of neurons with nocodazole significantly inhibited the movement of green fluorescent protein-SYNCRIP-positive granules, indicating that the transport of SYNCRIP-containing granules is dependent on microtubules. The distribution of SYNCRIP-containing granules overlapped with that of dendritic RNAs and elongation factor 1α. SYNCRIP was also found to be co-transported with green fluorescent protein-tagged human staufen1 and the 3′-untranslated region of inositol 1,4,5-trisphosphate receptor type 1 mRNA. These results suggest that SYNCRIP is transported within the dendrite as a component of mRNA granules and raise the possibility that mRNA turnover in mRNA granules and the regulation of local protein synthesis in neuronal dendrites may involve SYNCRIP.",
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AU - Fukatsu, Kazumi

AU - Mizutani, Akihiro

AU - Natsume, Tohru

AU - Iemura, Shuin Ichiro

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AU - Inoue, Takafumi

AU - Mikoshiba, Katsuhiko

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