An ultrastructural and carotenoid analysis of the red ventrum of the Japanese newt, Cynops pyrrhogaster

Kumi Matsui, Junsuke Marunouchi, Masahisa Nakamura

    Research output: Contribution to journalArticle

    13 Citations (Scopus)

    Abstract

    The ventral skin of the wild Japanese newt Cynops pyrrhogaster is creamy at metamorphosis, but turns red when mature. The color of the ventral skin of laboratory (lab)-reared newts stays yellow throughout their life. However, the mechanism for the red coloration of this animal still remains unknown. In this study, we have performed ultrastructural and carotenoid analyses of the red ventrum of wild and lab-reared Japanese newts. Using electron microscopy, we observed a number of xanthophores having ring carotenoid vesicles (rcv) and homogenous carotenoid granules (hcg) in the ventral red skin of the wild newt. In the skin, β-carotene and five other kinds of carotenoids were detected by thin-layer chromatography (TLC). In the ventral yellow skin of lab-reared newts, however, only β-carotene and three other kinds of carotenoids were found. The total amount of carotenoids in the red skin of the wild adult newt was six times more than that of the yellow skin of the lab-reared newt. Moreover, rcv were more abundant in xanthophores in red skin, but hcg were more abundant in yellow skin. These results, taken together, suggest that the presence of carotenoids in rcv in xanthophores is one of the critical factors for producing the red ventral coloration of the Japanese newt C. pyrrhogaster.

    Original languageEnglish
    Pages (from-to)265-272
    Number of pages8
    JournalPigment Cell Research
    Volume15
    Issue number4
    DOIs
    Publication statusPublished - 2002

    Fingerprint

    Salamandridae
    Carotenoids
    salamanders and newts
    skin (animal)
    carotenoids
    Skin
    carotenes
    color
    granules
    Skin Pigmentation
    Thin layer chromatography
    thin layer chromatography
    metamorphosis
    Thin Layer Chromatography
    electron microscopy
    Electron microscopy
    Electron Microscopy
    Animals

    Keywords

    • Carotenoid
    • Japanese newt
    • Ultrastructure
    • Xanthophore

    ASJC Scopus subject areas

    • Cell Biology
    • Agronomy and Crop Science
    • Plant Science
    • Clinical Biochemistry
    • Developmental Biology

    Cite this

    An ultrastructural and carotenoid analysis of the red ventrum of the Japanese newt, Cynops pyrrhogaster. / Matsui, Kumi; Marunouchi, Junsuke; Nakamura, Masahisa.

    In: Pigment Cell Research, Vol. 15, No. 4, 2002, p. 265-272.

    Research output: Contribution to journalArticle

    Matsui, Kumi ; Marunouchi, Junsuke ; Nakamura, Masahisa. / An ultrastructural and carotenoid analysis of the red ventrum of the Japanese newt, Cynops pyrrhogaster. In: Pigment Cell Research. 2002 ; Vol. 15, No. 4. pp. 265-272.
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