Analysis of binding energies between luciferin and luciferase adsorbed on Si surface by docking simulations

Katsuhiko Nishiyama, Takanobu Watanabe, Tadatsugu Hoshino, Iwao Ohdomari

    Research output: Contribution to journalArticle

    Abstract

    The binding energies between luciferin and luciferase adsorbed on a Si surface were estimated by docking simulations. These binding energies were more stable on a hydrophilic Si surface than on a hydrophobic Si surface, but their difference was small. Luciferase adsorbed more strongly on the hydrophobic Si surface than on the hydrophilic Si surface. The luciferase active site was not covered by the Si surface in this adsorption state. The hydrophobic surface would be suitable for the immobilization of luciferase both from the viewpoints of the binding of luciferin to luciferase and the adsorption of luciferase to the Si surface.

    Original languageEnglish
    Pages (from-to)148-150
    Number of pages3
    JournalChemical Physics Letters
    Volume439
    Issue number1-3
    DOIs
    Publication statusPublished - 2007 May 4

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    Binding energy
    Luciferases
    binding energy
    simulation
    Adsorption
    adsorption
    immobilization

    ASJC Scopus subject areas

    • Physical and Theoretical Chemistry
    • Spectroscopy
    • Atomic and Molecular Physics, and Optics
    • Surfaces and Interfaces
    • Condensed Matter Physics

    Cite this

    Analysis of binding energies between luciferin and luciferase adsorbed on Si surface by docking simulations. / Nishiyama, Katsuhiko; Watanabe, Takanobu; Hoshino, Tadatsugu; Ohdomari, Iwao.

    In: Chemical Physics Letters, Vol. 439, No. 1-3, 04.05.2007, p. 148-150.

    Research output: Contribution to journalArticle

    Nishiyama, Katsuhiko ; Watanabe, Takanobu ; Hoshino, Tadatsugu ; Ohdomari, Iwao. / Analysis of binding energies between luciferin and luciferase adsorbed on Si surface by docking simulations. In: Chemical Physics Letters. 2007 ; Vol. 439, No. 1-3. pp. 148-150.
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