Analysis of calcium signals in steering neuronal growth cones in vitro

Hiroki Akiyama, Hiroyuki Kamiguchi

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Calcium imaging allows us to measure the spatial and temporal changes in intracellular calcium concentration in living cells. Localized calcium elevation often functions as the polarizing signal during guided migration including axon guidance. In this chapter, we describe a protocol to quantitatively monitor the spatiotemporal dynamics of calcium signals in neuronal growth cones in the presence of an extracellular concentration gradient of axon guidance cue.

Original languageEnglish
Pages (from-to)17-27
Number of pages11
JournalMethods in Molecular Biology
Volume1162
DOIs
Publication statusPublished - 2014
Externally publishedYes

Fingerprint

Growth Cones
Calcium
Cues
In Vitro Techniques
Axon Guidance

Keywords

  • Axon guidance
  • Ca<sup>2+</sup> imaging
  • Chemotaxis
  • Growth cone
  • Turning assay

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Medicine(all)

Cite this

Analysis of calcium signals in steering neuronal growth cones in vitro. / Akiyama, Hiroki; Kamiguchi, Hiroyuki.

In: Methods in Molecular Biology, Vol. 1162, 2014, p. 17-27.

Research output: Contribution to journalArticle

Akiyama, Hiroki ; Kamiguchi, Hiroyuki. / Analysis of calcium signals in steering neuronal growth cones in vitro. In: Methods in Molecular Biology. 2014 ; Vol. 1162. pp. 17-27.
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