Analysis of DNA endoreplication in the brain neurons in the terrestrial slug, Limax valentianus.

R. Matsuo, Miki Yamagishi, Etsuro Ito

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

DNA endoreplication is the DNA synthesis without cell division, resulting in the generation of a nucleus containing a larger amount of genomic DNA compared to a normal diploid genome. There are many such giant neurons in the molluscan brain that are generated as a result of repeated endoreplication. However, it has been controversial whether the endoreplication is the whole genome replication (polyploidy) or the local amplification of the genes that are necessary for the neuron's function (polyteny/polysomy). Here in this study, we investigated these two possibilities by (1) immunohistochemical analysis of the distribution of 5'-bromodeoxyuridine incorporated into the nuclei of the brain neurons, and by (2) quantitative genomic PCR directed to two different genes expressed in specific brain regions. Our data supported the view that the DNA endoreplication is the whole genome replication rather than the local amplification of a specific genomic region.

Original languageEnglish
Pages (from-to)171-178
Number of pages8
JournalActa Biologica Hungarica
Volume63 Suppl 2
DOIs
Publication statusPublished - 2012
Externally publishedYes

Fingerprint

Endoreduplication
Gastropoda
slug
Neurons
brain
Brain
Genes
DNA
genomics
genome
Genome
amplification
Amplification
polyploidy
Polyploidy
gene
Gene Amplification
Bromodeoxyuridine
Diploidy
Cell Division

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Environmental Science(all)
  • Neurology

Cite this

Analysis of DNA endoreplication in the brain neurons in the terrestrial slug, Limax valentianus. / Matsuo, R.; Yamagishi, Miki; Ito, Etsuro.

In: Acta Biologica Hungarica, Vol. 63 Suppl 2, 2012, p. 171-178.

Research output: Contribution to journalArticle

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