Analysis of DNA methylation by bisulfite reaction in neural cells as an example of orexin neurons

Koji Hayakawa, Mitsuko Hirosawa, Kunio Shiota

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Epigenetic regulation provides the basis for embryonic development, cellular differentiation, and maintenance of cellular function. DNA methylation is involved in various biological phenomena through gene silencing, stabilizing chromosomal structure and suppressing the mobility of retrotransposons. Here, we describe the methods of analyzing DNA methylation by bisulfi te-DNA reactions and combined bisulfi te restriction analysis (COBRA), as an example study of orexin neurons. These methods are useful for basic and applied neurobiology.

Original languageEnglish
Title of host publicationNeuromethods
PublisherHumana Press Inc.
Pages55-63
Number of pages9
Volume105
DOIs
Publication statusPublished - 2016
Externally publishedYes

Publication series

NameNeuromethods
Volume105
ISSN (Print)08932336
ISSN (Electronic)19406045

Keywords

  • Bisulfite reaction
  • Bisulfite sequence
  • COBRA
  • DNA methylation
  • Hypothalamus
  • Orexin

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)
  • Neuroscience(all)
  • Psychiatry and Mental health

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