Abstract
Single-cell genomics has enabled the exploration of cellular diversity in environmental microbes. However, current genome sequencing techniques, which utilizes next-generation sequencing (NGS), typically require nanogram to microgram levels of input DNA sample. Since single bacterial cells contain only a few femtograms of DNA, we have to amplify their genomes to adequate amount for sequencing. We aimed to develop a novel system for precise and high throughput single-cell genomics, to elucidate environmental microbial diversity. In this study, we have developed droplet-based microfluidic system to produce the compartmentalized reaction vessels for single-cell genome sequencing.
Original language | English |
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Title of host publication | TRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems |
Publisher | Institute of Electrical and Electronics Engineers Inc. |
Pages | 634-637 |
Number of pages | 4 |
ISBN (Electronic) | 9781538627310 |
DOIs | |
Publication status | Published - 2017 Jul 26 |
Event | 19th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2017 - Kaohsiung, Taiwan, Province of China Duration: 2017 Jun 18 → 2017 Jun 22 |
Other
Other | 19th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2017 |
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Country | Taiwan, Province of China |
City | Kaohsiung |
Period | 17/6/18 → 17/6/22 |
Keywords
- Droplet
- Microfluidics
- NGS
- Single-cell genomics
ASJC Scopus subject areas
- Chemical Health and Safety
- Instrumentation
- Electrical and Electronic Engineering