Analysis of environmental bacteria at single-cell level

Masahito Hosokawa; Yohei Nishikawa; Masato Kogawa; Haruko Takeyama

doi:10.1109/TRANSDUCERS.2017.7994129

Analysis of environmental bacteria at single-cell level

Masahito Hosokawa, Yohei Nishikawa, Masato Kogawa, Haruko Takeyama

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

Single-cell genomics has enabled the exploration of cellular diversity in environmental microbes. However, current genome sequencing techniques, which utilizes next-generation sequencing (NGS), typically require nanogram to microgram levels of input DNA sample. Since single bacterial cells contain only a few femtograms of DNA, we have to amplify their genomes to adequate amount for sequencing. We aimed to develop a novel system for precise and high throughput single-cell genomics, to elucidate environmental microbial diversity. In this study, we have developed droplet-based microfluidic system to produce the compartmentalized reaction vessels for single-cell genome sequencing.

Original language	English
Title of host publication	TRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems
Publisher	Institute of Electrical and Electronics Engineers Inc.
Pages	634-637
Number of pages	4
ISBN (Electronic)	9781538627310
DOIs	https://doi.org/10.1109/TRANSDUCERS.2017.7994129
Publication status	Published - 2017 Jul 26
Event	19th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2017 - Kaohsiung, Taiwan, Province of China Duration: 2017 Jun 18 → 2017 Jun 22

Other

Other	19th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2017
Country	Taiwan, Province of China
City	Kaohsiung
Period	17/6/18 → 17/6/22

Keywords

Droplet
Microfluidics
NGS
Single-cell genomics

ASJC Scopus subject areas

Chemical Health and Safety
Instrumentation
Electrical and Electronic Engineering

Access to Document

10.1109/TRANSDUCERS.2017.7994129

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Analysis of environmental bacteria at single-cell level. / Hosokawa, Masahito; Nishikawa, Yohei; Kogawa, Masato ; Takeyama, Haruko.

TRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems. Institute of Electrical and Electronics Engineers Inc., 2017. p. 634-637 7994129.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Hosokawa, M, Nishikawa, Y, Kogawa, M & Takeyama, H 2017, Analysis of environmental bacteria at single-cell level. in TRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems., 7994129, Institute of Electrical and Electronics Engineers Inc., pp. 634-637, 19th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2017, Kaohsiung, Taiwan, Province of China, 17/6/18. https://doi.org/10.1109/TRANSDUCERS.2017.7994129

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