Analysis of ferrylHb radical reduction by L-tyrosine and its application to hemoglobin vesicles

Tomovasu Atoji, Hirotaka Yatami, Motonari Aihara, Eishun Tsuchida, Shinji Takeoka

    Research output: Chapter in Book/Report/Conference proceedingConference contribution

    Abstract

    We found that a mixed solution of methemoglobin (metHb) and L-tyrosine (L-Tyr) showed an effective H2O2 elimination ability by utilizing the peroxidase activity of metHb using L-Tyr as a substrate. The rapid radical disappearance and the appearance of metHb signal by L-Tyr addition were confirmed in the ESR measurement. In the mechanism, ferrylHb radicals are reduced to metHb by the electron donation from L-Tyr. From these results, stable H2O2 elimination by metHb/L-Tyr mixed solution would be due to the combination of peroxidase and pseudo-catalase activity of ferrylHb radicals. Furthermore, in the application to the Hb vesicles co-encapsulating metHb/L-Tyr, the 50 % metHb conversion from oxyHb in the vesicles was prolonged to 44 hr from 14hr of the Hb vesicles without metHb/L-Tyr.

    Original languageEnglish
    Title of host publicationPolymer Preprints, Japan
    Pages1923
    Number of pages1
    Volume55
    Edition1
    Publication statusPublished - 2006
    Event55th SPSJ Annual Meeting - Nagoya
    Duration: 2006 May 242006 May 26

    Other

    Other55th SPSJ Annual Meeting
    CityNagoya
    Period06/5/2406/5/26

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    Keywords

    • FerrylHb radical
    • Hemoglobin vesicles
    • L-Tyr
    • Methb
    • Peroxidase activity

    ASJC Scopus subject areas

    • Engineering(all)

    Cite this

    Atoji, T., Yatami, H., Aihara, M., Tsuchida, E., & Takeoka, S. (2006). Analysis of ferrylHb radical reduction by L-tyrosine and its application to hemoglobin vesicles. In Polymer Preprints, Japan (1 ed., Vol. 55, pp. 1923)