Analysis of Functional Groups of Porphyrins by Collision-Induced Dissociation Mass Spectrometry/Mass Spectrometry

Toshimichi Shibue, Hisashi Kambe, Hiroyuki Nishide, Yoko Ohashi

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Collision-induced dissociation mass spectrometry/mass spectrometry (CID-MS/MS) was effective for estimating the substituent groups of porphyrin. The substituents of the porphyrin plane, which form the pathway for an oxygen-binding reaction, are essential for its oxygen-binding affinity. The CID-MS/MS of 5, 1 0,1 5-tris(ο-pivalamidophenyl)monoaminoporphyrinatocobalt and 5, 10, 15, 20-tetrakis(o-pivalamidophenyl)porphyrinatocopper was measured, and their fragmentations were studied using Bj E-constant and B(l—E)1/2E-constant linked-scan techniques. These CID-MS/MS spectra clearly show that the substituent group represents the eliminations of the alkyl and acyl radicals from the molecular ions. Thus, the CID-MS/MS technique has been proven to be useful for characterizing porphyrin substituents.

Original languageEnglish
Pages (from-to)793-796
Number of pages4
JournalAnalytical Sciences
Volume11
Issue number5
DOIs
Publication statusPublished - 1995

Fingerprint

Porphyrins
Functional groups
Mass spectrometry
Oxygen
Ions

Keywords

  • B(l—E)/E-constant linked-scan
  • B/E-constant linked-scan
  • collision-induced dissociation mass spectrometry/mass spectrometry
  • mass spectrometry
  • Porphyrin

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Analysis of Functional Groups of Porphyrins by Collision-Induced Dissociation Mass Spectrometry/Mass Spectrometry. / Shibue, Toshimichi; Kambe, Hisashi; Nishide, Hiroyuki; Ohashi, Yoko.

In: Analytical Sciences, Vol. 11, No. 5, 1995, p. 793-796.

Research output: Contribution to journalArticle

@article{87e26cd428ce493ba611e9f866311e87,
title = "Analysis of Functional Groups of Porphyrins by Collision-Induced Dissociation Mass Spectrometry/Mass Spectrometry",
abstract = "Collision-induced dissociation mass spectrometry/mass spectrometry (CID-MS/MS) was effective for estimating the substituent groups of porphyrin. The substituents of the porphyrin plane, which form the pathway for an oxygen-binding reaction, are essential for its oxygen-binding affinity. The CID-MS/MS of 5, 1 0,1 5-tris(ο-pivalamidophenyl)monoaminoporphyrinatocobalt and 5, 10, 15, 20-tetrakis(o-pivalamidophenyl)porphyrinatocopper was measured, and their fragmentations were studied using Bj E-constant and B(l—E)1/2E-constant linked-scan techniques. These CID-MS/MS spectra clearly show that the substituent group represents the eliminations of the alkyl and acyl radicals from the molecular ions. Thus, the CID-MS/MS technique has been proven to be useful for characterizing porphyrin substituents.",
keywords = "B(l—E)/E-constant linked-scan, B/E-constant linked-scan, collision-induced dissociation mass spectrometry/mass spectrometry, mass spectrometry, Porphyrin",
author = "Toshimichi Shibue and Hisashi Kambe and Hiroyuki Nishide and Yoko Ohashi",
year = "1995",
doi = "10.2116/analsci.11.793",
language = "English",
volume = "11",
pages = "793--796",
journal = "Analytical Sciences",
issn = "0910-6340",
publisher = "Japan Society for Analytical Chemistry",
number = "5",

}

TY - JOUR

T1 - Analysis of Functional Groups of Porphyrins by Collision-Induced Dissociation Mass Spectrometry/Mass Spectrometry

AU - Shibue, Toshimichi

AU - Kambe, Hisashi

AU - Nishide, Hiroyuki

AU - Ohashi, Yoko

PY - 1995

Y1 - 1995

N2 - Collision-induced dissociation mass spectrometry/mass spectrometry (CID-MS/MS) was effective for estimating the substituent groups of porphyrin. The substituents of the porphyrin plane, which form the pathway for an oxygen-binding reaction, are essential for its oxygen-binding affinity. The CID-MS/MS of 5, 1 0,1 5-tris(ο-pivalamidophenyl)monoaminoporphyrinatocobalt and 5, 10, 15, 20-tetrakis(o-pivalamidophenyl)porphyrinatocopper was measured, and their fragmentations were studied using Bj E-constant and B(l—E)1/2E-constant linked-scan techniques. These CID-MS/MS spectra clearly show that the substituent group represents the eliminations of the alkyl and acyl radicals from the molecular ions. Thus, the CID-MS/MS technique has been proven to be useful for characterizing porphyrin substituents.

AB - Collision-induced dissociation mass spectrometry/mass spectrometry (CID-MS/MS) was effective for estimating the substituent groups of porphyrin. The substituents of the porphyrin plane, which form the pathway for an oxygen-binding reaction, are essential for its oxygen-binding affinity. The CID-MS/MS of 5, 1 0,1 5-tris(ο-pivalamidophenyl)monoaminoporphyrinatocobalt and 5, 10, 15, 20-tetrakis(o-pivalamidophenyl)porphyrinatocopper was measured, and their fragmentations were studied using Bj E-constant and B(l—E)1/2E-constant linked-scan techniques. These CID-MS/MS spectra clearly show that the substituent group represents the eliminations of the alkyl and acyl radicals from the molecular ions. Thus, the CID-MS/MS technique has been proven to be useful for characterizing porphyrin substituents.

KW - B(l—E)/E-constant linked-scan

KW - B/E-constant linked-scan

KW - collision-induced dissociation mass spectrometry/mass spectrometry

KW - mass spectrometry

KW - Porphyrin

UR - http://www.scopus.com/inward/record.url?scp=85011248466&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85011248466&partnerID=8YFLogxK

U2 - 10.2116/analsci.11.793

DO - 10.2116/analsci.11.793

M3 - Article

VL - 11

SP - 793

EP - 796

JO - Analytical Sciences

JF - Analytical Sciences

SN - 0910-6340

IS - 5

ER -