Analysis of histone modifications in neural cells as an example of orexin neurons

Koji Hayakawa, Chikako Yoneda, Ruiko Tani, Kunio Shiota

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Epigenetic regulation, which causes structural changes in chromatin in a locus-specific and/or genome-wide manner, is the basic mechanism for embryonic development, cellular differentiation, and maintenance of cellular function. Histone modifications, together with DNA methylation, represent the major epigenetic events. Here, we describe a chromatin immunoprecipitation (ChIP) method for the analysis of histone modifications, taking a study of orexin neurons as an example. This method can be used in basic and applied neurobiology.

Original languageEnglish
Title of host publicationNeuromethods
PublisherHumana Press Inc.
Pages139-147
Number of pages9
Volume105
DOIs
Publication statusPublished - 2016
Externally publishedYes

Publication series

NameNeuromethods
Volume105
ISSN (Print)08932336
ISSN (Electronic)19406045

Keywords

  • Chromatin immunoprecipitation
  • Histone acetylation
  • Histone modification
  • Hypothalamus
  • Orexin

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)
  • Neuroscience(all)
  • Psychiatry and Mental health

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  • Cite this

    Hayakawa, K., Yoneda, C., Tani, R., & Shiota, K. (2016). Analysis of histone modifications in neural cells as an example of orexin neurons. In Neuromethods (Vol. 105, pp. 139-147). (Neuromethods; Vol. 105). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-2754-8_9