Analysis of histone modifications in neural cells as an example of orexin neurons

Koji Hayakawa, Chikako Yoneda, Ruiko Tani, Kunio Shiota

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Epigenetic regulation, which causes structural changes in chromatin in a locus-specific and/or genome-wide manner, is the basic mechanism for embryonic development, cellular differentiation, and maintenance of cellular function. Histone modifications, together with DNA methylation, represent the major epigenetic events. Here, we describe a chromatin immunoprecipitation (ChIP) method for the analysis of histone modifications, taking a study of orexin neurons as an example. This method can be used in basic and applied neurobiology.

Original languageEnglish
Title of host publicationNeuromethods
PublisherHumana Press Inc.
Pages139-147
Number of pages9
Volume105
DOIs
Publication statusPublished - 2016
Externally publishedYes

Publication series

NameNeuromethods
Volume105
ISSN (Print)08932336
ISSN (Electronic)19406045

Fingerprint

Histone Code
Epigenomics
Histones
Neurons
Chromatin
Neurobiology
Chromatin Immunoprecipitation
DNA Methylation
Embryonic Development
Genes
Maintenance
Genome

Keywords

  • Chromatin immunoprecipitation
  • Histone acetylation
  • Histone modification
  • Hypothalamus
  • Orexin

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)
  • Neuroscience(all)
  • Psychiatry and Mental health

Cite this

Hayakawa, K., Yoneda, C., Tani, R., & Shiota, K. (2016). Analysis of histone modifications in neural cells as an example of orexin neurons. In Neuromethods (Vol. 105, pp. 139-147). (Neuromethods; Vol. 105). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-2754-8_9

Analysis of histone modifications in neural cells as an example of orexin neurons. / Hayakawa, Koji; Yoneda, Chikako; Tani, Ruiko; Shiota, Kunio.

Neuromethods. Vol. 105 Humana Press Inc., 2016. p. 139-147 (Neuromethods; Vol. 105).

Research output: Chapter in Book/Report/Conference proceedingChapter

Hayakawa, K, Yoneda, C, Tani, R & Shiota, K 2016, Analysis of histone modifications in neural cells as an example of orexin neurons. in Neuromethods. vol. 105, Neuromethods, vol. 105, Humana Press Inc., pp. 139-147. https://doi.org/10.1007/978-1-4939-2754-8_9
Hayakawa K, Yoneda C, Tani R, Shiota K. Analysis of histone modifications in neural cells as an example of orexin neurons. In Neuromethods. Vol. 105. Humana Press Inc. 2016. p. 139-147. (Neuromethods). https://doi.org/10.1007/978-1-4939-2754-8_9
Hayakawa, Koji ; Yoneda, Chikako ; Tani, Ruiko ; Shiota, Kunio. / Analysis of histone modifications in neural cells as an example of orexin neurons. Neuromethods. Vol. 105 Humana Press Inc., 2016. pp. 139-147 (Neuromethods).
@inbook{5111f1dc22ae4db0bc3715ba029bb19c,
title = "Analysis of histone modifications in neural cells as an example of orexin neurons",
abstract = "Epigenetic regulation, which causes structural changes in chromatin in a locus-specific and/or genome-wide manner, is the basic mechanism for embryonic development, cellular differentiation, and maintenance of cellular function. Histone modifications, together with DNA methylation, represent the major epigenetic events. Here, we describe a chromatin immunoprecipitation (ChIP) method for the analysis of histone modifications, taking a study of orexin neurons as an example. This method can be used in basic and applied neurobiology.",
keywords = "Chromatin immunoprecipitation, Histone acetylation, Histone modification, Hypothalamus, Orexin",
author = "Koji Hayakawa and Chikako Yoneda and Ruiko Tani and Kunio Shiota",
year = "2016",
doi = "10.1007/978-1-4939-2754-8_9",
language = "English",
volume = "105",
series = "Neuromethods",
publisher = "Humana Press Inc.",
pages = "139--147",
booktitle = "Neuromethods",

}

TY - CHAP

T1 - Analysis of histone modifications in neural cells as an example of orexin neurons

AU - Hayakawa, Koji

AU - Yoneda, Chikako

AU - Tani, Ruiko

AU - Shiota, Kunio

PY - 2016

Y1 - 2016

N2 - Epigenetic regulation, which causes structural changes in chromatin in a locus-specific and/or genome-wide manner, is the basic mechanism for embryonic development, cellular differentiation, and maintenance of cellular function. Histone modifications, together with DNA methylation, represent the major epigenetic events. Here, we describe a chromatin immunoprecipitation (ChIP) method for the analysis of histone modifications, taking a study of orexin neurons as an example. This method can be used in basic and applied neurobiology.

AB - Epigenetic regulation, which causes structural changes in chromatin in a locus-specific and/or genome-wide manner, is the basic mechanism for embryonic development, cellular differentiation, and maintenance of cellular function. Histone modifications, together with DNA methylation, represent the major epigenetic events. Here, we describe a chromatin immunoprecipitation (ChIP) method for the analysis of histone modifications, taking a study of orexin neurons as an example. This method can be used in basic and applied neurobiology.

KW - Chromatin immunoprecipitation

KW - Histone acetylation

KW - Histone modification

KW - Hypothalamus

KW - Orexin

UR - http://www.scopus.com/inward/record.url?scp=84944463207&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84944463207&partnerID=8YFLogxK

U2 - 10.1007/978-1-4939-2754-8_9

DO - 10.1007/978-1-4939-2754-8_9

M3 - Chapter

AN - SCOPUS:84944463207

VL - 105

T3 - Neuromethods

SP - 139

EP - 147

BT - Neuromethods

PB - Humana Press Inc.

ER -