Analysis of signal transmissions from suprachiasmatic nucleus in the rat brain using optical recordings

Takayoshi Hosono, Misato Nakano, Rie Terai, Tomoko Yagishita, Ryuhei Okuno, Kenzo Akazawa, Kazuyuki Kanosue

Research output: Contribution to journalArticlepeer-review

Abstract

Neuronal signal propagations from the optic nerve to the hypothalamus via the suprachias- matic nucleus (SCN), which works as the center for generating circadian rhythm in the body, were analyzed using two-dimensional optic recording with a voltage-sensitive dye. When electric stimulation on the optic chiasma (OX) was applied to a slice preparation of rat brain including SCN, signal propagations from the vetrolateral SCN to dorsomedian SCN were successfully recorded by the optical recording system. The signals propagated dorsally along the third ventricle. The optic signals disappeared in the low Ca2+ and high Mg2+ artificial cerebrospinal fluid, which blocks synaptic transmissions. Signal propagation from the SCN to the hypothalamus was synaptically mediated. Application of the NMDA receptor blocker decreased the responses, and the application of a vasoactive intestinal polypeptide (VIP) antagonist also weakened the response. These results indicate that the NMDA receptor and VIP are involved in signal propagation in the SCN.

Original languageEnglish
Pages (from-to)405-409
Number of pages5
JournalJapanese journal of medical electronics and biological engineering
Volume41
Issue number4
Publication statusPublished - 2003
Externally publishedYes

ASJC Scopus subject areas

  • Biomedical Engineering

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