Analysis of single-cell differences by use of an on-chip microculture system and optical trapping

Yuichi Wakamoto, Ippei Inoue, Hiroyuki Moriguchi, Kenji Yasuda

Research output: Contribution to journalArticle

76 Citations (Scopus)

Abstract

A method is described for continuous observation of isolated single cells that enables genetically identical cells to be compared; it uses an on-chip microculture system and optical tweezers. Photolithography is used to construct microchambers with 5-μm-high walls made of thick photoresist (SU-8) on the surface of a glass slide. These microchambers are connected by a channel through which cells are transported, by means of optical tweezers, from a cultivation microchamber to an analysis microchamber, or from the analysis microchamber to a waste microchamber. The microchambers are covered with a semi-permeable membrane to separate them from nutrient medium circulating through a "cover chamber" above. Differential analysis of isolated direct descendants of single cells showed that this system could be used to compare genetically identical cells under contamination-free conditions. It should thus help in the clarification of heterogeneous phenomena, for example unequal cell division and cell differentiation.

Original languageEnglish
Pages (from-to)276-281
Number of pages6
JournalAnalytical and Bioanalytical Chemistry
Volume371
Issue number2
DOIs
Publication statusPublished - 2001
Externally publishedYes

Fingerprint

Optical Tweezers
Single-Cell Analysis
Optical tweezers
Circulating media
Photolithography
Photoresists
Nutrients
Contamination
Cells
Membranes
Glass
Cell Division
Cell Differentiation
Observation
System-on-chip
Food

ASJC Scopus subject areas

  • Analytical Chemistry
  • Clinical Biochemistry
  • Biochemistry

Cite this

Analysis of single-cell differences by use of an on-chip microculture system and optical trapping. / Wakamoto, Yuichi; Inoue, Ippei; Moriguchi, Hiroyuki; Yasuda, Kenji.

In: Analytical and Bioanalytical Chemistry, Vol. 371, No. 2, 2001, p. 276-281.

Research output: Contribution to journalArticle

Wakamoto, Yuichi ; Inoue, Ippei ; Moriguchi, Hiroyuki ; Yasuda, Kenji. / Analysis of single-cell differences by use of an on-chip microculture system and optical trapping. In: Analytical and Bioanalytical Chemistry. 2001 ; Vol. 371, No. 2. pp. 276-281.
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