Anisotropic swelling behavior of the cornea

Toyoaki Matsuura, Hitoe Ikeda, Naokazu Idota, Ryuhei Motokawa, Yoshiaki Hara, Masahiko Annaka

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

The phase equilibrium property and structural and dynamical properties of pig cornea were studied by macroscopic observation of swelling behavior, dynamic light scattering (DLS), and small-angle X-ray scattering (SAXS) under various conditions. It was found that the corneal gel collapses into a compact state isotropically or anisotropically depending on the external conditions. The corneal gel collapses uniformly into a compact state at a temperature above 55 °C because of the denaturation of collagen, whereas it collapses along an axis parallel to the optic axis with increasing NaCl concentration. Anisotropic deswelling was also observed during desiccation. SAXS measurements revealed that the periodicity of the collagen fiber of the cornea does not change even at higher NaCl concentration, which indicates that hydration and dehydration resulting from changes in salt concentration simply cause swelling and deswelling of the glycosaminoglycan (GAG), which is located between the regular two-dimensional lattices of collagen fibers, which obliges the change in thickness. From observations of the dynamics of light scattered by the corneal gel, intensity autocorrelation functions that revealed two independent diffusion coefficients were obtained. Divergent behavior in the measured total scattered light intensities and diffusion coefficients with varying temperature was observed. That is, a slowing of the dynamic modes accompanied by increased "static" scattered intensities was observed. This is indicative of the occurrence of a phase transition as a function of temperature.

Original languageEnglish
Pages (from-to)16314-16322
Number of pages9
JournalJournal of Physical Chemistry B
Volume113
Issue number51
DOIs
Publication statusPublished - 2009 Dec 24
Externally publishedYes

Fingerprint

cornea
collagens
Collagen
swelling
Swelling
Gels
gels
X ray scattering
diffusion coefficient
Denaturation
fibers
swine
Fibers
biopolymer denaturation
Dynamic light scattering
Glycosaminoglycans
Dehydration
scattering
Autocorrelation
dehydration

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Materials Chemistry
  • Surfaces, Coatings and Films

Cite this

Matsuura, T., Ikeda, H., Idota, N., Motokawa, R., Hara, Y., & Annaka, M. (2009). Anisotropic swelling behavior of the cornea. Journal of Physical Chemistry B, 113(51), 16314-16322. https://doi.org/10.1021/jp907232h

Anisotropic swelling behavior of the cornea. / Matsuura, Toyoaki; Ikeda, Hitoe; Idota, Naokazu; Motokawa, Ryuhei; Hara, Yoshiaki; Annaka, Masahiko.

In: Journal of Physical Chemistry B, Vol. 113, No. 51, 24.12.2009, p. 16314-16322.

Research output: Contribution to journalArticle

Matsuura, T, Ikeda, H, Idota, N, Motokawa, R, Hara, Y & Annaka, M 2009, 'Anisotropic swelling behavior of the cornea', Journal of Physical Chemistry B, vol. 113, no. 51, pp. 16314-16322. https://doi.org/10.1021/jp907232h
Matsuura T, Ikeda H, Idota N, Motokawa R, Hara Y, Annaka M. Anisotropic swelling behavior of the cornea. Journal of Physical Chemistry B. 2009 Dec 24;113(51):16314-16322. https://doi.org/10.1021/jp907232h
Matsuura, Toyoaki ; Ikeda, Hitoe ; Idota, Naokazu ; Motokawa, Ryuhei ; Hara, Yoshiaki ; Annaka, Masahiko. / Anisotropic swelling behavior of the cornea. In: Journal of Physical Chemistry B. 2009 ; Vol. 113, No. 51. pp. 16314-16322.
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