Anomer selective formation of l-menthyl α-D-glucopyranoside by α- glucosidase-catalyzed reaction

H. Nakagawa, M. Yoshiyama, S. Shimura, Kotaro Kirimura, S. Usami

    Research output: Contribution to journalArticle

    16 Citations (Scopus)

    Abstract

    l-Menthol was glucosylated by the α-glucosidase (EC 3.2.1.20) of Saccharomyces cerevisiae using maltose as glucosyl donor. When 50 mg of l- menthol and 1 M maltose in 10 mM citrate-phosphate buffer (pH 7.0) were incubated for 24 h at 30°C, a menthylglucoside was selectively obtained as a product. The molar conversion yield based on supplied menthol was 4.5%. The product was identified as l-menthyl α-D-glucopyranoside (α-MenG) by 13C- NMR analysis.

    Original languageEnglish
    Pages (from-to)1914-1915
    Number of pages2
    JournalBioscience, Biotechnology and Biochemistry
    Volume60
    Issue number11
    Publication statusPublished - 1996

    Fingerprint

    Glucosidases
    Menthol
    menthol
    glucosidases
    Maltose
    maltose
    alpha-glucosidase
    Citric Acid
    citrates
    Yeast
    Saccharomyces cerevisiae
    Buffers
    buffers
    Phosphates
    Nuclear magnetic resonance
    phosphates

    Keywords

    • α-glucosidase
    • α-glucoside formation
    • menthylglucoside
    • transglucosylation

    ASJC Scopus subject areas

    • Food Science
    • Biochemistry, Genetics and Molecular Biology(all)
    • Biochemistry
    • Biotechnology
    • Chemistry (miscellaneous)
    • Applied Microbiology and Biotechnology

    Cite this

    Anomer selective formation of l-menthyl α-D-glucopyranoside by α- glucosidase-catalyzed reaction. / Nakagawa, H.; Yoshiyama, M.; Shimura, S.; Kirimura, Kotaro; Usami, S.

    In: Bioscience, Biotechnology and Biochemistry, Vol. 60, No. 11, 1996, p. 1914-1915.

    Research output: Contribution to journalArticle

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    AU - Yoshiyama, M.

    AU - Shimura, S.

    AU - Kirimura, Kotaro

    AU - Usami, S.

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    AB - l-Menthol was glucosylated by the α-glucosidase (EC 3.2.1.20) of Saccharomyces cerevisiae using maltose as glucosyl donor. When 50 mg of l- menthol and 1 M maltose in 10 mM citrate-phosphate buffer (pH 7.0) were incubated for 24 h at 30°C, a menthylglucoside was selectively obtained as a product. The molar conversion yield based on supplied menthol was 4.5%. The product was identified as l-menthyl α-D-glucopyranoside (α-MenG) by 13C- NMR analysis.

    KW - α-glucosidase

    KW - α-glucoside formation

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    KW - transglucosylation

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