Anomer selective formation of l-menthyl α-D-glucopyranoside by α-glucosidase-catalyzed reaction

Hiroyuki Nakagawa, Masaaki Yoshiyama, Susumu Shimura, Kohtaro Kirimura, Shoji Usami

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16 Citations (Scopus)

Abstract

L-Menthol was glucosylated by the α-glucosidase (EC 3.2.1.20) of Saccharomyces cerevisiae using maltose as glucosyl donor. When 50 mg of l-menthol and 1M maltose in 10 mM citrate–phosphate buffer (pH 7.0) were incubated for 24 h at 30°C, a menthylglucoside was selectively obtained as a product. The molar conversion yield based on supplied menthol was 4.5%. The product was identified as l-menthyl α-D-glucopyranoside (α-MenG) by 13C-NMR analysis.

Original languageEnglish
Pages (from-to)1914-1915
Number of pages2
JournalBioscience, Biotechnology and Biochemistry
Volume60
Issue number11
DOIs
Publication statusPublished - 1996 Jan

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Keywords

  • Menthylglucoside
  • Transglucosylation
  • α-glucosidase
  • α-glucoside formation

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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