Anomer-selective glucosylation of l-menthol using lyophilized cells of Saccharomyces cerevisiae

Kentaro Noguchi; Hiroyuki Nakagawa; Masaaki Yoshiyama; Susumu Shimura; Kohtaro Kirimura; Shoji Usami

doi:10.1016/S0922-338X(98)80090-1

Anomer-selective glucosylation of l-menthol using lyophilized cells of Saccharomyces cerevisiae

Kentaro Noguchi, Hiroyuki Nakagawa, Masaaki Yoshiyama, Susumu Shimura, Kohtaro Kirimura, Shoji Usami^*

^*Corresponding author for this work

School of Advanced Science and Engineering

Research output: Contribution to journal › Article › peer-review

21 Citations (Scopus)

Abstract

A novel glucoside of l-menthol was synthesized from l-menthol and maltose by reaction with the lyophilized cells of Saccharomyces cerevisiae. Conditions for the synthetic reaction were examined and optimized. When 50 mg of l-menthol and 1.4 M maltose in 10 mM citrate-10 mM phosphate buffer (pH 7.0) were incubated with the lyophilized cells for 96 h at 40°C, l-menthyl α-D-glucopyranoside (α-MenG) was selectively obtained as a product, and the maximum molar conversion yield based on l-menthol supplied reached 19.8%.

Original language	English
Pages (from-to)	436-438
Number of pages	3
Journal	Journal of Fermentation and Bioengineering
Volume	85
Issue number	4
DOIs	https://doi.org/10.1016/S0922-338X(98)80090-1
Publication status	Published - 1998 Jan 1

Keywords

Glucoside
Menthol
Saccharomyces cerevisiae
Transglucosylation
α- glucosidase

ASJC Scopus subject areas

Biotechnology
Applied Microbiology and Biotechnology

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10.1016/S0922-338X(98)80090-1

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title = "Anomer-selective glucosylation of l-menthol using lyophilized cells of Saccharomyces cerevisiae",

abstract = "A novel glucoside of l-menthol was synthesized from l-menthol and maltose by reaction with the lyophilized cells of Saccharomyces cerevisiae. Conditions for the synthetic reaction were examined and optimized. When 50 mg of l-menthol and 1.4 M maltose in 10 mM citrate-10 mM phosphate buffer (pH 7.0) were incubated with the lyophilized cells for 96 h at 40°C, l-menthyl α-D-glucopyranoside (α-MenG) was selectively obtained as a product, and the maximum molar conversion yield based on l-menthol supplied reached 19.8%.",

keywords = "Glucoside, Menthol, Saccharomyces cerevisiae, Transglucosylation, α- glucosidase",

author = "Kentaro Noguchi and Hiroyuki Nakagawa and Masaaki Yoshiyama and Susumu Shimura and Kohtaro Kirimura and Shoji Usami",

year = "1998",

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doi = "10.1016/S0922-338X(98)80090-1",

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T1 - Anomer-selective glucosylation of l-menthol using lyophilized cells of Saccharomyces cerevisiae

AU - Noguchi, Kentaro

AU - Nakagawa, Hiroyuki

AU - Yoshiyama, Masaaki

AU - Shimura, Susumu

AU - Kirimura, Kohtaro

AU - Usami, Shoji

PY - 1998/1/1

Y1 - 1998/1/1

N2 - A novel glucoside of l-menthol was synthesized from l-menthol and maltose by reaction with the lyophilized cells of Saccharomyces cerevisiae. Conditions for the synthetic reaction were examined and optimized. When 50 mg of l-menthol and 1.4 M maltose in 10 mM citrate-10 mM phosphate buffer (pH 7.0) were incubated with the lyophilized cells for 96 h at 40°C, l-menthyl α-D-glucopyranoside (α-MenG) was selectively obtained as a product, and the maximum molar conversion yield based on l-menthol supplied reached 19.8%.

AB - A novel glucoside of l-menthol was synthesized from l-menthol and maltose by reaction with the lyophilized cells of Saccharomyces cerevisiae. Conditions for the synthetic reaction were examined and optimized. When 50 mg of l-menthol and 1.4 M maltose in 10 mM citrate-10 mM phosphate buffer (pH 7.0) were incubated with the lyophilized cells for 96 h at 40°C, l-menthyl α-D-glucopyranoside (α-MenG) was selectively obtained as a product, and the maximum molar conversion yield based on l-menthol supplied reached 19.8%.

KW - Glucoside

KW - Menthol

KW - Saccharomyces cerevisiae

KW - Transglucosylation

KW - α- glucosidase

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DO - 10.1016/S0922-338X(98)80090-1

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VL - 85

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JO - Journal of Bioscience and Bioengineering

JF - Journal of Bioscience and Bioengineering

IS - 4

ER -

Anomer-selective glucosylation of l-menthol using lyophilized cells of Saccharomyces cerevisiae

Abstract

Keywords

ASJC Scopus subject areas

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