Anomer-selective glucosylation of l-menthol using lyophilized cells of Saccharomyces cerevisiae

Kentaro Noguchi, Hiroyuki Nakagawa, Masaaki Yoshiyama, Susumu Shimura, Kohtaro Kirimura, Shoji Usami

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19 Citations (Scopus)

Abstract

A novel glucoside of l-menthol was synthesized from l-menthol and maltose by reaction with the lyophilized cells of Saccharomyces cerevisiae. Conditions for the synthetic reaction were examined and optimized. When 50 mg of l-menthol and 1.4 M maltose in 10 mM citrate-10 mM phosphate buffer (pH 7.0) were incubated with the lyophilized cells for 96 h at 40°C, l-menthyl α-D-glucopyranoside (α-MenG) was selectively obtained as a product, and the maximum molar conversion yield based on l-menthol supplied reached 19.8%.

Original languageEnglish
Pages (from-to)436-438
Number of pages3
JournalJournal of Fermentation and Bioengineering
Volume85
Issue number4
DOIs
Publication statusPublished - 1998 Jan 1

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Keywords

  • Glucoside
  • Menthol
  • Saccharomyces cerevisiae
  • Transglucosylation
  • α- glucosidase

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

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