Antigen exposure in the late light period induces severe symptoms of food allergy in an OVA-allergic mouse model

Kana Tanabe, Eri Kitagawa, Misaki Wada, Atsushi Haraguchi, Kanami Orihara, Yu Tahara, Atsuhito Nakao, Shigenobu Shibata

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The mammalian circadian clock controls many physiological processes that include immune responses and allergic reactions. Several studies have investigated the circadian regulation of intestinal permeability and tight junctions known to be affected by cytokines. However, the contribution of circadian clock to food allergy symptoms remains unclear. Therefore, we investigated the role of the circadian clock in determining the severity of food allergies. We prepared an ovalbumin food allergy mouse model, and orally administered ovalbumin either late in the light or late in the dark period under light-dark cycle. The light period group showed higher allergic diarrhea and weight loss than the dark period group. The production of type 2 cytokines, IL-13 and IL-5, from the mesenteric lymph nodes and ovalbumin absorption was higher in the light period group than in the dark period group. Compared to the dark period group, the mRNA expression levels of the tight junction proteins were lower in the light period group. We have demonstrated that increased production of type 2 cytokines and intestinal permeability in the light period induced severe food allergy symptoms. Our results suggest that the time of food antigen intake might affect the determination of the severity of food allergy symptoms.

Original languageEnglish
Article number14424
JournalScientific Reports
Volume5
DOIs
Publication statusPublished - 2015 Sep 30

Fingerprint

Food Hypersensitivity
Circadian Clocks
Ovalbumin
Light
Antigens
Cytokines
Permeability
Physiological Phenomena
Tight Junction Proteins
Interleukin-13
Tight Junctions
Interleukin-5
Photoperiod
Weight Loss
Diarrhea
Hypersensitivity
Eating
Lymph Nodes
Messenger RNA

ASJC Scopus subject areas

  • General

Cite this

Antigen exposure in the late light period induces severe symptoms of food allergy in an OVA-allergic mouse model. / Tanabe, Kana; Kitagawa, Eri; Wada, Misaki; Haraguchi, Atsushi; Orihara, Kanami; Tahara, Yu; Nakao, Atsuhito; Shibata, Shigenobu.

In: Scientific Reports, Vol. 5, 14424, 30.09.2015.

Research output: Contribution to journalArticle

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